黄芪蜜炙前后多糖的结构变化研究  

before and after Processing with Honey Study on Structural Changes of Polysaccharides in Huangqi(Astragali Radix)

作　　者：聂紫璇 常源 缪翼翔 史辑[1] 刘蓬蓬[1] NIE Zixuan;CHANG Yuan;MIAO Yixiang;SHI Ji;LIU Pengpeng(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Key Laboratory of Processing Technology and Principle,National Administration of Traditional Chinese Medicine,Technology Innovation Center for Traditional Chinese Medicine Processing of Liaoning Province,Dalian 116600,Liaoning,China)

机构地区：[1]辽宁中医药大学药学院,国家中医药管理局中药炮制工艺原理重点研究室,辽宁省中药炮制专业技术创新中心,辽宁大连116600

出　　处：《中华中医药学刊》2024年第12期129-132,I0049,I0050,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(82003945);辽宁省自然科学基金项目(2020-BS-163);辽宁中医药大学招聘录用高层次人才科研启动基金项目(201911)。

摘　　要：采用水提醇沉法提取生、炙黄芪中的多糖成分,并经DEAE-52层析柱和HW-65F凝胶柱色谱分离纯化。采用高效液相凝胶渗透色谱法测定多糖的纯度和分子量,采用离子色谱技术对多糖中的单糖组分进行检测分析,采用红外光谱技术对糖链结构进行测定。结果表明生、炙黄芪经提取、分离、纯化后分别主要获得均一多糖APS-1和均一多糖HAPS-1。APS-1和HAPS-1的分子质量分别为7.839kDa和21.36kDa,且APS-1和HAPS-1均由阿拉伯糖、半乳糖、葡萄糖、木糖和甘露糖组成,APS-1摩尔比率为8.00∶3.00∶41.00∶1∶2.00,HAPS-1摩尔比率为12.73∶3.68∶48.92∶1∶1.21。APS-1和HAPS-1均具有O-H、C-H、C=O官能团,此外APS-1还具有C-O官能团。黄芪蜜炙后,多糖的分子量和单糖组成的比例均发生了变化。研究可为从炮制前后多糖变化的角度来阐释炙黄芪增效提供参考依据。The polysaccharides from raw and honey-processed Huangqi(Astragali Radix)were extracted by water extraction and alcohol precipitation.The polysaccharides were separated and purified by DEAE-52 chromatography and HW-65F gel column.APS-1 and HAPS-1 were obtained from raw and honey-processed Huangqi(Astragali Radix),respectively.The purity and molecular weights of APS-1 and HAPS-1 were determined by high performance liquid gel permeation chromatography.The monosaccharides in polysaccharides were analyzed by electrochemical detector.The sugar chain structure of APS-1 and HAPS-1 was analyzed by infrared spectroscopy.The results show that APS-1 and HAPS-1 are the main polysaccharides in raw and honey-processed Huangqi(Astragali Radix).The molecular weights(Mw)of APS-I and HAPS-I were 7.839 kDa and 21.36 kDa,respectively.It is shown that APS-I and HAPS-I are consisted of arabinose,galactose,glucose,xylose,mannose and levulose,in a molar ratio of 8.00∶3.00∶41.00∶1∶2.00 and 12.73∶3.68∶48.92∶1∶1.21,respectively.Both APS-1 and HAPS 1 have functional groups O-H,C-H and C=O.APS-1 also has functional groups C-O.The molecular weight of polysaccharide and the proportion of monosaccharide composition are changed after honey processing.This study can provide a reference for interpreting the synergistic effect of Huangqi(Astragali Radix)from the perspective of polysaccharide changes before and after processing.

关 键 词：黄芪多糖 炙黄芪多糖 分离 纯化 结构鉴定 

分 类 号：R282.71[医药卫生—中药学]