针刺对动脉粥样硬化家兔肝脏胆固醇逆转运受体表达的影响  

Effects of Acupuncture on Expression of Cholesterol Reverse Transport Receptors in Liver of Atherosclerotic Rabbits

作　　者：栾海燕 佟晓哲 张邵宁 赵莹莹 姜凤伟 李慧 韩婧姝 成泽东[2] 李菁媛 LUAN Haiyan;TONG Xiaozhe;ZHANG Shaoning;ZHAO Yingying;JIANG Fengwei;LI Hui;HAN Jingshu;CHENG Zedong;LI Jingyuan(The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,Liaoning,China;Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)

机构地区：[1]锦州医科大学附属第一医院,辽宁锦州121001 [2]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2024年第12期180-184,I0061,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81674078);辽宁省博士科研启动基金计划项目(2021-BS-268)。

摘　　要：目的观察电针干预“内关”“关元”“足三里”穴对动脉粥样硬化(atherosclerosis,AS)家兔肝脏胆固醇逆转运受体ABCA1、ABCG1、SR-BⅠ基因及蛋白表达的影响,探索电针治疗AS的可能机制。方法将26只雄性新西兰兔随机选取7只为阴性对照组,19只为造模组,阴性对照组喂以普通饲料,造模组采用高脂喂养与颈总动脉球囊损伤术相结合的方法制备AS模型,确认造模成功后,将造模组家兔随机分为AS模型组(6只)、电针治疗组(6只)和西药治疗组(6只)。AS模型组不给予治疗,电针治疗组对家兔“内关”“足三里”“关元”穴进行电针治疗,输出波形为疏密波,电流为1 mA,频率为4 Hz/20 Hz,1次/d,每次留针20 min,6 d为1个疗程,治疗4个疗程;西药治疗组将阿托伐他汀钙片制成悬浊液给予家兔灌胃治疗,剂量为1 mg/(kg·d),1次/d,每次20 mL,6 d为1个疗程,治疗4个疗程。疗程结束后,取家兔肝脏组织,HE染色观察肝脏病理形态学;RT-PCR法检测三磷酸腺苷结合盒转运蛋白A1(ATP-binding cassette trans-porter A1,ABCA1)、三磷酸腺苷结合盒转运蛋白G1(ATP-binding cassette transporter G1,ABCG1)和B类Ⅰ型清道夫受体(scavenger receptor B typeⅠ,SR-BⅠ)mRNA表达水平;WB法检测ABCA1、ABCG1、SR-BⅠ的蛋白表达水平。结果与阴性对照组比较,AS模型组肝脏组织结构紊乱,肝血窦受压变窄。肝细胞体积增大,胞质疏松,可见脂肪空泡。与AS模型组比较,电针治疗组与西药治疗组肝细胞呈条索状排列,细胞索间见肝血窦,肝细胞圆形,肝脏结构恢复接近正常,脂肪空泡明显减轻;与阴性对照组比较,AS模型组肝脏中ABCA1、ABCG1、SR-BⅠmRNA和蛋白含量均明显降低,差异有统计学意义(P<0.001);与AS模型组比较,电针治疗组和西药治疗组家兔肝脏中ABCA1、ABCG1、SR-BⅠmR-NA和蛋白含量均明显升高,差异有统计学意义(P<0.001);与电针治疗组比较,西药治疗组家兔肝脏中ABCA1、AB-CG1 mRNA�Objective To observe the effects of electroacupuncture intervention at“Neiguan(PC6)”,“Guanyuan(ST25)”and“Zusanli”(ST36)on the gene and protein expressions of liver cholesterol reversal transport receptor ABCA1,ABCG1 and SR-BⅠin rabbits with atherosclerosis(AS),and explore the possible mechanism of electroacupuncture treatment of AS.Methods 26 male New Zealand rabbits were randomly selected as the negative control group(7 rabbits)and the modeling group(19 rabbits).The negative control group was fed with regular feed,and the modeling group was prepared using a combination of high-fat feed-ing and carotid artery balloon injury surgery.After successful modeling,the modeling group rabbits were randomly divided into AS model group(6 rabbits),electroacupuncture treatment group(6 rabbits)and Western medicine treatment group(6 rabbits).The AS model group was not treated,while the electroacupuncture treatment group used electroacupuncture at the rabbit's“Neiguan”(PC6),“Zusanli”(ST36)and“Guanyuan”(ST25).The output waveform was a dense wave,with a current of 1 mA and a fre-quency of 4 Hz/20 Hz,once a day.The needle was left for 20 minutes for each time and 6 days was considered as a course of treatment,with 4 courses of treatment.In the western medicine treatment group,Atorvastatin Calcium Tablets were made into a suspension and given to rabbits by gavage.The dose was 1 mg/(kg·d),once a day,20 mL for each time.Six days was a course of treatment,with four courses of treatment.After the treatment,rabbit liver tissues were collected and HE staining was used to observe the liver pathophysiology.The mRNA expression levels of ABCA1,ABCG1 and SR-BⅠwere detected by RT-PCR.The protein expression levels of ABCA1,ABCG1 and SR-BⅠwere detected by Western Blot.Results Compared with the nega-tive control group,the liver tissue structure of the AS model group was disordered,and the hepatic sinus was compressed and nar-rowed.The volume of liver cells increased,the cytoplasm was loose,and fat vacuoles were obser

关 键 词：动脉粥样硬化 针刺 胆固醇逆转运受体 

分 类 号：R245.31[医药卫生—针灸推拿学]