牛膝及炮制品标准汤剂的量值传递研究  

Study on Quantity Value Transfer of Standard Decoction in Raw and Processed Niuxi(Achyranthis Bidentatae Radix)

作　　者：赵新梅 贾豪 杨春静[2] 雷敬卫[1] 谢彩侠[1] 王晓亚 孙天成 ZHAO Xinmei;JIA Hao;YANG Chunjing;LEI Jingwei;XIE Caixia;WANG Xiaoya;SUN Tiancheng(Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;The Third Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450046,Henan,China;Luohe Medical College,Luohe 462002,Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南中医药大学第三附属医院,河南郑州450046 [3]漯河医学高等专科学校,河南漯河462002

出　　处：《中华中医药学刊》2024年第12期245-251,I0066,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：河南省中医药科学研究专项(2022ZY1156)。

摘　　要：目的建立牛膝生品、酒牛膝和盐牛膝标准汤剂高效液相色谱法(HPLC)指纹图谱,研究指标成分的传递情况。方法采用VenusilC18(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,柱温为30℃,检测波长为230nm,280nm,进样量为20μL,流速为0.8mL·min-1。采用《中药色谱指纹图谱相似度评价系统(2012版)》进行相似度评价,确定共有峰;测定指标成分含量,计算转移率,出膏率。结果45批样品的相似度均在0.934以上,45批样品共标定10个共有峰,指认出7个色谱峰,分别为5-羟基糠醛,水龙骨素B,β-蜕皮甾酮,25R-牛膝甾酮,25S-牛膝甾酮,人参皂苷R0,竹节参皂苷Ⅳa;牛膝生品标准汤剂中7个成分平均转移率分别为291.53%,28.90%,56.74%,5461%,53.01%,64.28%,61.87%,酒牛膝标准汤剂中7个成分平均转移率分别为192.21%,61.16%,56.10%,5469%,51.80%,58.06%,43.70%,盐牛膝标准汤剂中7个成分平均转移率分别为111.29%,53.59%,62.47%,6084%,57.77%,50.20%,48.69%,牛膝生品、酒牛膝、盐牛膝标准汤剂平均出膏率分别为35.06%,34.02%,36.32%。结论建立的指纹图谱方法稳定可靠,能充分展示指标成分在样品制备过程中转移情况,为其不同炮制品的临床应用及含牛膝经典名方复方制剂研究提供参考。Objective To establish efficient high-performance liquid chromatography(HPLC)fingerprints for the standard de-coction of raw and processed Niuxi(Achyranthis Bidentatae Radix)and study the transfer of index components.Methods The chromatographic column was Venusil C18(250 mm×4.6 mm,5μm)column.The column temperature was 30℃,and the detec-tion wavelength was 230,280 nm.The injection volume was 20μL and the flow rate was 0.8 mL·min-1.Chromatographic Fin-gerprint Similarity Evaluation System of Traditional Chinese Medicines(2012 Edition)was used to evaluate the similarity and de-termine the common peak.The contents of index components were determined,and the transfer rate and extraction rate were cal-culated.Results The similarity of all 45 batches of samples was above 0.934.Forty-five batches of samples were calibrated with 10 common peaks,referring to the recognition of 7 peaks,namely 5-hydroxyfurfural,polypodine B,β-ecdysone,25R-inokos-teron,25S-inokosteron,ginsenoside R0 and chikusetsu saponinⅣa.The average transfer rates of the seven components in the standard decoction of the raw and processed products of Niuxi(Achyranthis Bidentatae Radix)were 291.53%,28.90%,5674%,54.61%,53.01%,64.28%,61.87%and 192.21%,61.16%,56.10%,54.69%,51.80%,58.06%,43.70%and 111.29%,53.59%,62.47%,60.84%,57.77%,50.20%,48.69%.The average extraction rate of the standard decoction of the raw and processed products of Niuxi(Achyranthis Bidentatae Radix)of standard decoction was 35.06%,34.02%and 3632%.Conclusion The fingerprint method is stable and reliable,and it can fully demonstrate the transfer of index components during sample preparation,which can provides a reference for the study of classic prescriptions and compound preparation contai-ning Niuxi(Achyranthis Bidentatae Radix).

关 键 词：牛膝 酒牛膝 盐牛膝 标准汤剂 指纹图谱 量值传递 

分 类 号：R285.5[医药卫生—中药学]