阻塞性睡眠呼吸暂停低通气综合征患者血清外泌体对A549肺癌细胞生长侵袭的影响  

Effect of serum exosomes from obstructive sleep apnea-hypopnea syndrome patients on growth and invasion of A549 cells

作　　者：阿力木江·排尔哈提 包雅丽 郭海[3] 邓淇 姚巧玲 Alimujiang Paierhati;BAO Yali;GUO Hai;DENG Qi;YAO Qiaoling(Department of Physiology,School of Basic Medical Sciences,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi830011,China;Department of Pathophysiology,School of Basic Medical Sciences,Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi830011,China;Department of Anesthesiology,the First Affiliated Hospital of Xinjiang Medical University,Xinjiang Uygur Autonomous Region,Urumqi830011,China)

机构地区：[1]新疆医科大学基础医学院生理学教研室,新疆乌鲁木齐830011 [2]新疆医科大学基础医学院病理生理学教研室,新疆乌鲁木齐830011 [3]新疆医科大学第一附属医院麻醉科,新疆乌鲁木齐830011

出　　处：《中国医药导报》2024年第26期1-8,共8页China Medical Herald

基　　金：国家自然科学基金资助项目(82260513)。

摘　　要：目的探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者血清外泌体对A549肺癌细胞生长侵袭的影响。方法选择新疆医科大学第一附属医院2023年8月至2024年2月收治的21例OSAHS患者作为试验组,体检的11名健康志愿者作为对照组。收集OSAHS患者和健康志愿者的血清,提取血清外泌体,将OSAHS患者血清外泌体与A549细胞共孵育视为OSAHS组,将健康志愿者血清外泌体与A549细胞共孵育作为HC组。采用CCK8实验检测肿瘤细胞的活力;采用Transwell实验和细胞划痕实验检测A549细胞的侵袭和迁移能力;采用AnnexinV-PE/TAAD双染法检测对A549细胞凋亡的作用;采用Western blot法检测A549细胞中E-钙黏蛋白、N-钙黏蛋白和基质金属蛋白酶9(MMP-9)的表达水平。结果与HC组比较,OSAHS组细胞活力升高(P<0.01)。与HC组比较,OSAHS组血清外泌体可增加A549细胞的侵袭和迁移能力(P<0.01)。OSAHS组MMP-9的表达水平高于HC组(P<0.05)。OSAHS组中A549细胞的凋亡率低于HC组(P<0.05)。结论OSAHS患者血清外泌体可能促进人A549肺癌细胞的生长侵袭。Objective To investigate the effect of serum exosomes from obstructive sleep apnea-hypopnea syndrome(OSAHS)patients on growth and invasion of A549 lung cancer cells.Methods A total of 21 patients with OSAHS admitted to the First Affiliated Hospital of Xinjiang Medical University from August 2023 to February 2024 were selected as the experimental group,and 11 healthy volunteers who underwent physical examinations were selected as the control group.Their serum and extracted serum exosomes from OSAHS patients and healthy volunteers were collected.OSAHS patients’serum exosomes co-cultured with A549 cells were regarded as OSAHS group,and healthy volunteers’serum exosomes co-cultured with A549 cells were regarded as HC group.CCK assay was used to detect tumor cell activity.Transwell assay and cell scratch assay were used to detect the invasion and migration ability of A549 cells.The AnnexinV-PE/TAAD apoptosis detection kit was used to detect the apoptosis of A549 cells.Western blot was used to detect the expression levels of E-cadherin,N-cadherin,and matrix metalloproteinase 9(MMP-9)in A549 cells.Results Compared with the HC group,the cell viability of OSAHS group was increased(P<0.01).Compared with the HC group,the invasion and migration ability of A549 cells were increased by serum exosomes in OSAHS group(P<0.01).The expression level of MMP-9 in OSAHS group was higher than that in HC group(P<0.05).The apoptosis rate of A549 cells in the OSAHS group was lower than that in the HC group(P<0.05).Conclusion Serum exosomes from OSAHS patients may promote the growth and invasion of A549 human lung cancer cells.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 A549肺癌细胞 生长 侵袭 

分 类 号：R332[医药卫生—人体生理学]