孟德尔随机化探索绿茶摄入量及消费频率与2型糖尿病发病风险之间的关联  

Exploration on the association between green tea intake and consumption frequency with the risk of developing diabetes mellitus type 2 using Mendelian randomization

作　　者：杨文菲 蒋新军 张翔 叶彩花 周启威 YANG Wenfei;JIANG Xinjun;ZHANG Xiang;YE Caihua;ZHOU Qiwei(International Nursing School,Hainan Medical University,Hainan Province,Haikou571199,China;Department of Nursing,the First Affiliated Hospital of Hainan Medical University,Hainan Province,Haikou571000,China)

机构地区：[1]海南医科大学国际护理学院,海南海口571199 [2]海南医科大学第一附属医院护理部,海南海口571000

出　　处：《中国医药导报》2024年第27期15-21,共7页China Medical Herald

基　　金：国家自然科学基金青年科学基金资助项目(82304262)。

摘　　要：目的采用两样本孟德尔随机化分析探讨绿茶摄入量及消费频率与2型糖尿病之间的因果关系,旨在为两者之间的潜在关联提供更可靠的证据。方法使用GWAS数据库数据,采用逆方差加权法(IVW)作为主要分析方法,用MR-Egger回归法、加权中位数法作为补充。敏感性分析中,采用Cochran’s Q进行异质性检验,并通过MR-Egger回归法截距和MR-PRESSO方法评估多效性。结果IVW结果显示,绿茶摄入量与2型糖尿病存在负向因果关系(OR=0.996,95%CI:0.994~0.999,P=0.005);加权中位数法、MR-Egger回归法与IVW方向一致,分别为(OR=0.993,95%CI:0.988~0.997,P=0.007)和(OR=0.995,95%CI:0.992~0.998,P=0.001)。同样,绿茶消费频率也显示与2型糖尿病存在负向因果关系(OR=0.857,95%CI:0.781~0.940,P=0.001),加权中位数(OR=0.992,95%CI:0.668~1.470,P=0.972)、MR-Egger与(OR=0.875,95%CI:0.773~0.991,P=0.036)与IVW方向一致。敏感性分析中均P>0.05,提示选定的单核苷酸多态性均不存在水平多效性和异质性。反向孟德尔随机化分析结果显示,2型糖尿病与绿茶摄入量和消费频率之间均未存在显著关联(P>0.05),提示2型糖尿病和绿茶摄入量和消费频率之间不存在反向因果关联。结论较高的绿茶摄入和消费频率可能有效降低2型糖尿病的发生风险。Objective To explore the causal relationship between green tea intake and consumption frequency and diabetes mellitus type 2 using the two-sample Mendelian randomization,aiming to provide more robust evidence for their potential association.Methods Using GWAS database data,inverse variance weighted(IVW)was used as the main analysis method,supplemented by MR-Egger regression method and weighted median method.In sensitivity analysis,Cochran’s Q was used to test for heterogeneity testing,and pleiotropy was assessed using the MR-Egger regression method intercept and MR-PRESSO method.Results The IVW showed a significant negative causal association between green tea intake and diabetes mellitus type 2(OR=0.996,95%CI:0.994-0.999,P=0.005).The weighted median method(OR=0.875,95%CI:0.773-0.991,P=0.036)and MR-Egger regression method were consistent with IVW direction,which were(OR=0.993,95%CI:0.988-0.997,P=0.007)and(OR=0.995,95%CI:0.992-0.998,P=0.001).Similarly,green tea consumption frequency also showed a negative association with diabetes mellitus type 2(OR=0.857,95%CI:0.781-0.940,P=0.001).The weighted median method and MR-Egger regression method(OR=0.992,95%CI:0.668-1.470,P=0.972)were consistent with IVW direction,respectively.Sensitivity analysis showed that all P>0.05 for all the selected single nucleotide polymorphisms did not have horizontal pleiotropy and heterogeneity.Reverse Mendelian randomization analysis showed no significant association between diabetes mellitus type 2 and green tea intake and consumption frequency(P>0.05),indicating that there was no reverse causal association between diabetes mellitus type 2 and green tea intake and consumption frequency.Conclusion Higher green tea intake and consumption frequency may be effective in reducing the risk of diabetes mellitus type 2.

关 键 词：绿茶摄入量 绿茶消费频率 2型糖尿病 孟德尔随机化 

分 类 号：R587.1[医药卫生—内分泌]