磺胺二甲嘧啶在许氏平鲉体内的富集与代谢  

Enrichment and metabolism of sulfamethazine in rockfish(Sebastes schlegelii)

作　　者：孙艺鸣 刘思宇 勇艳华 刘杨 赵洪霞[4] 陈思育 曲宝成 SUN Yiming;LIU Siyu;YONG Yanhua;LIU Yang;ZHAO Hongxia;CHEN Siyu;QU Baocheng(College of Marine Science and Environment,Dalian Ocean University,Dalian 116023,China;Key Laboratory of Environment Controlled Aquaculture,Ministry of Education(Dalian Ocean University),Dalian 116023,China;Dalian Testing and Certification Technical Service Center,Dalian 116630,China;School of Environmental Science&Technology,Dalian University of Technology,Dalian 116023,China)

机构地区：[1]大连海洋大学海洋科技与环境学院,辽宁大连116023 [2]设施渔业教育部重点实验室(大连海洋大学),辽宁大连116023 [3]大连市检验检测认证技术服务中心,辽宁大连116630 [4]大连理工大学环境学院,辽宁大连116023

出　　处：《大连海洋大学学报》2024年第5期766-772,共7页Journal of Dalian Ocean University

基　　金：大连海洋大学博士启动项目;辽宁省教育厅办公室2023年国家及省级大学生创新项目(S202310158002)。

摘　　要：为研究抗生素在海水鱼中的迁移转化情况,以评价抗生素的生态风险,通过液相色谱串联质谱定量分析及高分辨质谱检测分析方法,开展了磺胺二甲嘧啶(SMZ)在许氏平鲉(Sebastes schlegelii)(体质量为16.80 g±5.46 g)鱼体内的生物富集规律及代谢路径研究。结果表明:SMZ在环境水体暴露至第3天时鱼体内的富集浓度达到了整个暴露期的最高水平;在整个药物暴露过程中,鱼体内SMZ的最大富集浓度与药物浓度的高、低无明显的变化趋势差异;在最佳富集期内,SMZ在鱼体内富集趋势由高至低依次为鳃>肌肉>肝脏;代谢分析显示,SMZ在许氏平鲉体内的代谢产物有N^(4)-乙酰化磺胺二甲嘧啶和2-氨基-4,6-二甲基嘧啶两种,主要的代谢路径为SMZ乙酰化加成,以及与2-氨基-4,6-二甲基嘧啶相连的N-S键断裂。研究表明,海水中的磺胺类抗生素能够迁移至鱼体内主要组织器官,乙酰化加成和N—S键断裂是SMZ在许氏平鲉体内的主要代谢方式。In order to evaluate the transport and transformation of antibiotics in marine fish and evaluate the ecological risk of antibiotics,the bioconcentration pattern and metabolic pathway of sulfamethazine(SMZ)were detected in rockfish Sebastes schlegelii reared in a 30 L container and exposed to 0(control group),10,and 100μg/L SMZ at water temperature of(20±0.5)℃by liquid chromatography tandem mass spectrometry quantitative analysis and high-resolution mass spectrometry detection and analysis.The results showed that the maximal enrichment concentration of SMZ was observed in the fish exposed to SMZ in the third day during the whole exposure period,without significant difference between the maximum concentration of SMZ and the high and low concentrations of SMZ in fish.During the optimal enrichment period,the enrichment trend of SMZ in the fish was described as gill>muscle>liver.Metabolic analysis showed that the metabolites of SMZ in the test fish were N^(4)-acetylated SMZ and 2-amino-4,6-dimethylpyrimidine.The main metabolic pathways were SMZ acetylation addition and N-S bond cleavage linked to 2-amino-4,6-dimethylpyrimidine.In conclusion,sulfonamides antibiotics can migrate to the main tissues and organs of marine fish,and the main metabolic pathways of SMZ in Sebastes schlegelii are acetylation addition and N-S bond breakage.

关 键 词：许氏平鲉 磺胺二甲嘧啶 生物富集 代谢 

分 类 号：S967.9[农业科学—水产养殖]