虾夷扇贝响应高静水压胁迫的转录组分析  

Transcriptome analysis of Yesso scallop(Mizuhopecten yessoensis)exposed to high hydrostatic pressure stress

作　　者：范琛革 王许波 毛俊霞[1] 田莹[1] 郝振林[1] 宋坚[1] 尹东红[1] 常亚青[1] FAN Chenge;WANG Xubo;MAO Junxia;TIAN Ying;HAO Zhenlin;SONG Jian;YIN Donghong;CHANG Yaqing(Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]大连海洋大学农业农村部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2024年第5期781-787,共7页Journal of Dalian Ocean University

基　　金：国家重点研发计划项目(2022YFD2400302);大连市“揭榜挂帅”科技攻关项目(2021JB11SN035)。

摘　　要：为了研究虾夷扇贝(Mizuhopecten yessoensis)在静水压力胁迫条件下的生物学响应机理,采用转录组测序技术对受高静水压胁迫的虾夷扇贝(壳长为75.64 mm±2.15 mm)闭壳肌、鳃和外套膜进行了转录组学分析。结果表明:转录组筛选到闭壳肌中上调基因130个,下调基因150个;鳃中上调基因314个,下调基因390个;外套膜中上调基因314个,下调基因390个;GO富集分析发现,被注释的差异基因主要与参与细胞凋亡的半胱氨酸型内肽酶活性和抑制剂活性、细胞器膜、固有免疫应答有关;KEGG通路分析显示,差异表达基因多富集在抗原处理和呈递、细胞凋亡、内质网蛋白质加工和MAPK信号通路中。研究表明,虾夷扇贝在静水压力胁迫后,其差异表达基因主要富集在信号传导和免疫等通路中,这些发现为后续生物响应高压环境的研究提供了有益的数据和参考。To investigate the biological response mechanisms of the Yesso scallop(Mizuhopecten yessoensis)under static hydraulic pressure stress,the transcriptomes of the adductor muscle,gills,and mantle were analyzed in the Yesso scallops with shell length of(75.64 mm±2.15 mm)in a FH-100M manual hydrostatic press with a volume of 2 L at a high pressure of 25 MPa(equivalent to 2500 m water depth)by transcriptome sequencing technology.The results showed that 130 upregulated genes and 150 downregulated genes were identified in the adductor muscle,314 upregulated genes and 390 downregulated genes in the gills,and 314 upregulated genes and 390 downregulated genes in the mantle by transcriptome screening.GO enrichment analysis revealed that the annotated differentially expressed genes were found to be primarily associated with activities related to cysteine-type endopeptidase and inhibitor activities involved in apoptosis,organellar membranes,and innate immune response.KEGG pathway analysis showed that the differentially expressed genes were involved in antigen processing and presentation,apoptosis,endoplasmic reticulum protein processing,and the MAPK signaling pathway.It was found that the differentially expressed genes in the Yesso scallop exposed to static hydraulic pressure stress were primarily enriched in pathways related to signal transduction and immunity.The findings provide valuable data and references for subsequent research.

关 键 词：虾夷扇贝 高静水压力 转录组分析 差异表达基因 

分 类 号：S917.4[农业科学—水产科学]