磺胺甲恶唑/甲氧苄啶对固相反硝化脱氮影响及机制  

Effect and mechanism of sulfamethoxazole/trimethoprim on nitrogen removal by solid-phase denitrification

作　　者：张艳杰 王欢 董伟羊 闫国凯 常洋[1,3] 王海燕[1,3] 凌宇 魏有芳 田梓杨 ZHANG Yan-jie;WANG Huan;DONG Wei-yang;YAN Guo-kai;CHANG Yang;WANG Hai-yan;LING Yu;WEI You-fang;TIAN Zi-yang(State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 10012,China;College of Water Sciences,Beijing Normal University,Beijing 100875,China;Research Center of Environmental Pollution Control Technology,Chinese Research Academy of Environmental Sciences,Beijing 10012,China)

机构地区：[1]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [2]北京师范大学水科学研究院,北京100875 [3]中国环境科学研究院环境污染控制工程技术研究中心,北京100012

出　　处：《中国环境科学》2024年第12期6681-6690,共10页China Environmental Science

基　　金：宁夏回族自治区重点研发计划资助项目(2023BEG02055-02);中央财政科技计划结余经费专项资助项目(2021-JY-33)。

摘　　要：污水处理厂尾水中存在的抗生素磺胺甲恶唑(SMX)和甲氧苄啶(TMP)是否会影响植物碳源固相反硝化(SPD)技术的脱氮效果,以及影响机制尚不明晰.建立了3个连续流玉米芯SPD(CC-SPD)反应器,对比了反应器的脱氮性能,并通过宏基因组方法分析了影响机制.结果表明:(1)3个反应器平均出水NO_(3)^(–)-N浓度分别为(4.09±0.38)mg/L(RS)、(5.01±0.44)mg/L(RT)和(3.53±0.35)mg/L(RC).50μg/L SMX/TMP对CC-SPD反应器的脱氮性能并无显著影响;(2)SMX/TMP会显著改变微生物的群落结构,但是3个反应器主导的细菌门均为Proteobacteria、Bacteroidetes和Actinobacteria;(3)代谢是微生物群落的主要功能,微生物会增加环境信息处理和细胞过程功能的相对丰度来适应SMX/TMP;(4)50μg/L SMX/TMP并未显著影响与电子供体生成相关的酶和反硝化基因的相对丰度.It is unclear whether the antibiotics(i.e.sulfamethoxazole(SMX)and trimethoprim(TMP))in the tail water of wastewater treatment plants would affect the nitrogen removal performance of plant carbon source supported solid-phase denitrification(SPD)technology and its mechanism.Three continuous-flow corncob-SPD(CC-SPD)reactors were established,the nitrogen removal performance of the reactors was compared,and the influence mechanism was analyzed by the metagenomic method.The results showed that:(1)The average effluent NO_(3)^(-)-N concentrations of the three reactors were(4.09±0.38)mg/L(RS)(5.01±0.44)mg/L(RT)and(3.53±0.35)mg/L(RC),respectively.50μg/L SMX/TMP had no significant effect on the nitrogen removal performance of the CC-SPD reactor;(2)SMX/TMP could significantly change the microbial community structure,but the dominant bacteria in the reactor were Proteobacteria,Bacteroidetes,and Actinobacteria.Metabolism was the main function of the microbial community,and microorganisms improved the relative abundances of functions about environmental information processing and cellular processes to adapt to SMX/TMP.50μg/L SMX/TMP did not significantly affect the relative abundances of enzymes related to electron donor generation and denitrification genes.

关 键 词：固相反硝化 磺胺甲恶唑(Sulfamethoxazole SMX) 甲氧苄啶(Trimethoprim TMP) 玉米芯 电子供体 

分 类 号：X703[环境科学与工程—环境工程]