Anammox污泥中氮代谢与重金属抗性基因的关联性  

Correlation of nitrogen metabolism and heavy metal resistance genes in anammox sludges

作　　者：瞿才燕 唐溪 成思源 关惠泽 唐崇俭 QU Cai-yan;TANG Xi;CHENG Si-yuan;GUAN Hui-ze;TANG Chong-jian(National Engineering Research Center for Control and Treatment of Heavy Metal Pollution,School of Metallurgy and Environment,Central South University,Changsha 410083,China)

机构地区：[1]中南大学冶金与环境学院,国家重金属污染防治工程技术研究中心,湖南长沙410083

出　　处：《中国环境科学》2024年第12期6721-6728,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(U21A20294)。

摘　　要：为揭示不同聚集形态污泥中氮代谢和重金属抗性之间的相互作用,基于宏基因组分析了颗粒污泥、絮状污泥、生物膜中氮代谢基因和重金属抗性基因的丰度分布及关联性.基因丰度分析揭示了颗粒污泥中氮代谢基因总丰度和重金属抗性基因总丰度均高于絮状污泥(分别高24.5%和5.5%)和生物膜(分别高57.9%和16.7%),表明颗粒污泥具有最高的氮代谢潜力和重金属抗性.相关性网络分析进一步显示颗粒污泥网络的平均度分别比絮状污泥网络和生物膜网络高63.0%和54.4%,表明颗粒污泥中氮代谢基因和重金属抗性基因之间相关性最强.因此,颗粒污泥是厌氧氨氧化工艺处理重金属氨氮废水的最佳污泥聚集形态.To elucidate the interaction between nitrogen metabolism and heavy metal resistance in different sludge aggregates,we investigated gene abundance and correlations of nitrogen metabolism genes and heavy metal resistance genes.The gene abundance analysis revealed that the total abundance of nitrogen metabolism genes and heavy metal resistance genes in granules was higher than in flocs(by 24.5% and 5.5%,respectively) and biofilm(by 57.9% and 16.7%,respectively),indicating that granules exhibit the highest potential for nitrogen metabolism and heavy metal resistance.Further,the network analysis showed that the average degree of the granule network was 63.0% and 54.4% higher than in floc network and biofilm network,respectively,highlighting the strongest correlation between nitrogen metabolism genes and heavy metal resistance genes in granules.Therefore,the granule is the optimal sludge aggregate for anammox-based process in treating ammonia-rich wastewater containing heavy metals.

关 键 词：重金属抗性 氮代谢 厌氧氨氧化 污泥形态 

分 类 号：X703[环境科学与工程—环境工程]