黄淮肉羊IGF 1基因序列特征、组织表达及其与产肉性状的相关性分析  

Sequence Characteristics,Tissue Expression of IGF 1 Gene and Correlation with Production Trait in Huanghuai Sheep

作　　者：李君 宋梦柯 高福现 韩万里 韩浩园 施会彬 权凯 LI Jun;SONG Mengke;GAO Fuxian;HAN Wanli;HAN Haoyuan;SHI Huibin;QUAN Kai(College of Animal Science and Technology,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;College of Animal Science and Technology,Northeast Agricultural University,Harbin 150081,China)

机构地区：[1]河南牧业经济学院动物科技学院,郑州450046 [2]东北农业大学动物科学技术学院,哈尔滨150081

出　　处：《中国畜牧兽医》2024年第12期5380-5391,共12页China Animal Husbandry & Veterinary Medicine

基　　金：河南省重点研发与推广专项(科技攻关)(232102110007);河南省自然科学基金项目(242300420148);河南省现代农业产业技术体系建设专项资金(HARS-22-15-S)。

摘　　要：【目的】研究黄淮肉羊胰岛素样生长因子1(insulin like growth factor 1,IGF1)基因序列特征、组织表达规律,并对背最长肌中IGF 1基因的表达量与产肉性状进行相关性分析。【方法】选取3、9、18月龄的黄淮肉羊公羊各3只,屠宰后迅速采集半腱肌、股四头肌、臂三头肌、背最长肌、皮下脂肪、瘤胃、肾脏、脾脏、肝脏、睾丸和小肠组织样品并测定其产肉性能。对黄淮肉羊IGF 1基因进行克隆测序,并进行生物信息学分析,利用实时荧光定量PCR技术检测其在黄淮肉羊同一年龄(9月龄)各组织以及不同年龄阶段(3、9、18月龄)背最长肌中的mRNA水平,利用皮尔逊相关系数分析IGF 1基因的表达量与产肉性状的相关性。【结果】克隆获得黄淮肉羊IGF 1基因CDS序列465 bp,编码154个氨基酸,与山羊的亲缘关系最近。黄淮肉羊IGF1蛋白为亲水性不稳定蛋白,无跨膜结构,存在信号肽,有29个潜在的磷酸化位点。黄淮肉羊IGF1蛋白二级结构主要含有无规则卷曲(54.55%)和α-螺旋(29.87%),三级结构预测结果与二级结构一致。IGF1蛋白与IGF1R、IGFBP3、IGF2等存在蛋白互作关系。实时荧光定量PCR结果显示,IGF 1基因在黄淮肉羊各组织中均有表达,在肝脏组织中表达量最高,在臂三头肌中表达量最低。IGF 1基因在3月龄黄淮肉羊背最长肌中表达量最高,显著高于9和18月龄(P<0.05),而9月龄的表达量又显著低于18月龄(P<0.05)。相关性分析结果表明,IGF 1基因表达量与生长发育和肉品质指标无显著相关(P>0.05);体高、体长、胸围、管围、体重和胴体重之间呈显著或极显著正相关(P<0.05;P<0.01);屠宰率、肉色、大理石纹、眼肌面积之间呈显著或极显著正相关(P<0.05;P<0.01)。【结论】本研究成功克隆了黄淮肉羊IGF 1基因CDS区序列,IGF1蛋白属于亲水不稳定碱性蛋白,主要在肝脏中表达,在背最长肌中的表达趋势表现为随着月龄增长先降后�【Objective】This study was aimed to research the sequence characteristics and tissue expression patterns of insulin like growth factor 1(IGF1)gene in Huanghuai sheep,and conduct correlation analysis between the expression of IGF 1 gene in longissimus dorsi muscle and meat production traits.【Method】The 9 male Huanghuai sheep at the ages of 3,9,and 18 months each were selected.The meat production performance were determined and semitendinosus,quadriceps femoris,triceps brachii,longissimus dorsi muscle,subcutaneous fat,rumen,kidney,spleen,liver,testis and small intestine tissue samples were collected after slaughter.the IGF 1 gene of Huanghuai sheep was cloned and sequenced,and analyzed by bioinformatics analysis.The mRNA levels of IGF 1 gene in various tissues at 9-month-old and in the longissimus dorsi muscle at different age stages were detected by Real-time quantitative PCR,and the correlation between IGF 1 expression and meat production traits were analyzed through Pearson correlation coefficient.【Result】The CDS region size of IGF 1 gene in Huanghuai sheep was 465 bp,encoding 154 amino acids,indicating the closest genetic relationship with Capra hircus.IGF1 protein of Huanghuai sheep was a hydrophilic and unstable protein with no transmembrane structure.It contained signal peptides and had 29 potential phosphorylation sites.The secondary structure of IGF1 protein in Huanghuai sheep mainly contained random coil(54.55%)and alpha helix(29.87%),and the predicted results of the tertiary structure were consistent with the secondary structure.There were interactions between IGF1 and IGF1R,IGFBP3,IGF2 and other proteins.The results of Real-time quantitative PCR showed that IGF 1 gene was widely expressed in the tissues of Huanghuai sheep,with the highest expression in liver and the lowest expression in triceps brachii.The expression level of IGF 1 gene was highest in the longissimus dorsi muscle of 3-month-old Huanghuai sheep,significantly higher than that of 9-month-old and 18-month-old sheep(P<0.05),while

关 键 词：黄淮肉羊 IGF 1基因 组织表达 相关性 

分 类 号：S826[农业科学—畜牧学]