应用实验小动物高频超声评价小鼠2K1C肾动脉狭窄模型的研究  

Evaluation of mouse 2K1C renal artery stenosis model by high-frequency ultrasound in experimental small animals

作　　者：黄松 刘丽娜 高秋月 许晴 HUANG Song;LIU Lina;GAO Qiuyue;XU Qing(State-owned Assets and Laboratory Management Office,Capital Medical University,Beijing 100069,China;Core Facilities Centre,Capital Medical University,Beijing 100069,China;Department of Physiology and Pathophysiology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,China)

机构地区：[1]首都医科大学国有资产与实验室管理处,北京100069 [2]首都医科大学中心实验室,北京100069 [3]首都医科大学基础医学院生理与病理生理学学系,北京100069

出　　处：《中国现代医生》2024年第34期58-62,共5页China Modern Doctor

摘　　要：目的 探讨应用实验小动物高频超声评估小鼠肾动脉狭窄(renal artery stenosis,RAS)模型的方法。方法 选用C57B1/6J雄性小鼠,使用银夹夹闭左侧肾动脉制备RAS模型(RAS组,n=5),假手术组不夹闭左侧肾动脉(n=5)。术后3d开始测量血压。术后4周,应用实验小动物高频超声影像系统于胸骨旁左室短轴乳头肌水平记录左心室运动曲线,测量小鼠左心室前壁和后壁厚度,计算左心室质量、射血分数及左心室短轴缩短率;测量肾脏形态学参数(肾脏长度、宽度、厚度及肾皮质厚度)和肾动脉血管壁厚度、阻力指数;二维斑点追踪技术评价两组小鼠右侧肾动脉血管扩张性及血管壁整体径向应变。术后4周观察两组小鼠肾脏病理。结果 与假手术组相比,RAS组小鼠的收缩压显著升高,左心室前后壁厚度及左心室质量均显著增加(P<0.05)。与假手术组相比,RAS组小鼠的左侧肾脏长度、宽度、厚度及肾皮质厚度均显著减小(P<0.05),右侧肾脏代偿性增大、肾皮质厚度增加,右侧肾动脉血管壁显著增厚,右侧肾动脉血管扩张性和血管整体径向应变均显著降低(P<0.05)。结论 应用实验小动物高频超声可无创检测小鼠RAS模型的心脏、肾脏、肾血管损伤,并可分析肾动脉应变等生物力学指标,为小鼠RAS模型检测提供新的方法。Objective To investigate the method of evaluating renal artery stenosis(RAS)model in mice by high-frequency ultrasound in experimental small animals.Methods C57B1/6J male mice were selected and left renal artery was clamped with silver clip to prepare RAS model(RAS group,n=5),while left renal artery was not clamped in sham operation group(n=5).Systolic blood pressure was measured 3 days after surgery.Four weeks after surgery,the left ventricular movement curve was recorded at the level of parasternal left ventricular brachyaxis papillary muscle by high-frequency ultrasound of experimental small animals,mice left ventricular anterior wall and posterior wall thickness were measured,left ventricular mass,ejection fraction and left ventricular short axis reduced rate were calculate.The renal morphological parameters(kidney length,width,thickness and cortical thickness),the vascular wall thickness and resistance index of renal artery were measured.The right renal artery distensibility and global radial strain of the vessel wall were evaluated by two-dimensional dot tracking technique.Renal pathology was observed 4 weeks after surgery.Results Compared with sham operation group,systolic blood pressure,left ventricular anterior wall and posterior wall thickness and left ventricular mass were significantly increased in RAS group(P˂0.05).Compared with sham operation group,the length,width,thickness and cortical thickness of left kidney in RAS group were significantly reduced(P˂0.05).In the right kidney,the compensation increased,the thickness of the renal cortex increased,vascular wall of right renal artery was significantly thickened,distensibility and global radial strain of right renal artery were significantly reduced(P˂0.05).Conclusion The application of high-frequency ultrasound in experimental small animals can noninvasively detect the damage of the heart,kidney and renal vessels in RAS model,and analyze the biomechanical indexes such as renal artery strain,providing a new method for the detection of mouse RAS mo

关 键 词：两肾一夹模型 实验小动物高频超声 二维斑点追踪技术 无创检测 小鼠 

分 类 号：R544.14[医药卫生—心血管疾病]