湖南省岳阳地区无偿献血人群Mur血型抗原基因检测及抗体筛查  

Mur blood group antigen gene testing and antibody screening among voluntary blood donors in Yueyang,Hunan Province

作　　者：苏湘晖 周砺宏 向雅香 谭涛 SU Xianghui;ZHOU Lihong;XIANG Yaxiang;TAN Tao(Yueyang Central Blood Station,Yueyang,Hunan 414000,China)

机构地区：[1]岳阳市中心血站,湖南岳阳414000

出　　处：《现代医药卫生》2024年第23期4023-4025,4030,共4页Journal of Modern Medicine & Health

摘　　要：目的了解湖南省岳阳地区无偿献血人群Mur血型抗原分布频率及抗-Mur发生的概率,为临床输血安全提供保障。方法选取2023年1—6月该站无偿献血者标本4660人份,其中用聚合酶链反应-序列特异性引物检测Mur抗原标本437人份,用血清学方法检测抗-Mur标本4223份,有凝集反应的标本再进行红细胞抗体鉴定并测定效价;对2例Mur抗原进行基因测序。结果4660人份献血者标本Mur抗原分布频率为3.66%(16/437),抗-Mur发生率为0.17%(7/4223),抗体类型均为免疫球蛋白M型;Mur抗原基因测序结果均符合国际输血协会Mur抗原分型。结论湖南省岳阳地区献血人群Mur抗原和抗-Mur分布均有一定的频率,为确保临床输血安全和提高新生儿溶血病的实验诊断,不规则抗体检测试剂中应增加Mur抗原红细胞,可使用分子生物诊断试剂检测Mur抗原,为保障临床输血安全,必要时建立稀有血型库。Objective To investigate the distribution frequency of Mur blood group antigens and the incidence of anti-Mur among voluntary blood donors in Yueyang area of Hunan Province,so as to provide guarantee for the safety of clinical blood transfusion.Methods A total of 4660 voluntary blood donors in this station were selected from January to June 2023.Among them,437 samples were tested for Mur antigen by polymerase chain reaction-sequence specific primers,and 4223 samples were tested for anti-Mur by serological method.The samples with agglutination reaction were identified by red blood cell antibody and the titer was determined.Mur antigen gene sequencing was performed in two cases.Results The distribution frequency of Mur antigen was 3.66%(16/437),the incidence of anti-Mur was 0.17%(7/4223)in 4660 blood donors,and the antibody type was immunoglobulin M type.The sequencing results of the Mur antigen gene were consistent with Mur antigen of the International Society of Blood Transfusion.Conclusion The distribution of Mur antigen and anti-Mur in blood donors in Yueyang,Hunan Province has a certain frequency.In order to ensure the safety of clinical blood transfusion and improve the experimental diagnosis of hemolytic disease of newborn,Mur antigen red blood cells should be added,which can be detected by molecular biological diagnostic reagents.In order to ensure the safety of clinical blood transfusion,rare blood group library should be established when necessary.

关 键 词：Mur抗原 抗-MUR 基因 供血者 普查 湖南 

分 类 号：R193.3[医药卫生—卫生事业管理] R446.6[医药卫生—公共卫生与预防医学]