地奥心血康对动脉粥样硬化模型小鼠肠道菌群的影响  

Effect of Di’ao Xinxuekang on the Gut Microbiota in Atherosclerosis Model Mice

作　　者：李璐瑶 陈华[1] 瞿礼萍[1] 王加伟 李薇[1] 梁纪懿 邹文俊[1] LI Luyao;CHEN Hua;QU Liping;WANG Jiawei;LI Wei;LIANG Jiyi;ZOU Wenjun(State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137 Sichuan,China)

机构地区：[1]成都中医药大学西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中药新药与临床药理》2024年第12期1835-1841,共7页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金青年科学基金项目(82004175)。

摘　　要：目的 观察地奥心血康对动脉粥样硬化(AS)模型小鼠肠道菌群的影响。方法 采用高脂饮食饲喂ApoE^(-/-)小鼠建立AS模型,同期灌胃给予地奥心血康(80、160 mg·kg^(-1))、辛伐他汀(1.3 mg·kg^(-1)),于18周后测定小鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;油红O染色法观察主动脉斑块及肝脏脂滴形成;16SrRNA微生物多样性分析小鼠粪便菌群变化。结果与模型组比较,地奥心血康高剂量组和辛伐他汀组小鼠血清TC、TG、LDL-C水平明显降低(P<0.05,P<0.01),HDL-C明显升高(P<0.05,P<0.01);主动脉斑块及肝脏脂滴形成均得到不同程度的抑制(P<0.05,P<0.01)。α和β多样性分析显示,各组样本点能够显著分离。与对照组比较,模型组Akkermansia菌群丰度明显降低(P<0.01),给予地奥心血康可升高AS小鼠Akkermansia菌群丰度(P<0.05),且与TC、TG、LDL-C和肝脏脂滴面积等指标呈负相关(P<0.05,P<0.01)。结论 地奥心血康的降脂和抗AS作用可能与调节肠道菌群失衡,提高益生菌Akkermansia丰度有关。Objective To observe the effect of Di’ao Xinxuekang(XXK)on gut microbiota in atherosclerosis(AS)model mice.Methods ApoE-/-mice were fed a high-fat diet to establish the AS model,concurrently administered with XXK(80,160 mg·kg^(-1))and simvastatin(1.3 mg·kg^(-1)).After 18 weeks of the treatment,serum levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were measured in mice.Oil Red O staining was used to observe the formation of aortic plaque and hepatic lipid droplets.16S rRNA microbiota diversity analysis was performed to study changes of fecal microbiota in mice.Results Comparison with the model group,serum levels of TC,TG,and LDL-C in XXK high-dose group and simvastatin group were significantly reduced(P<0.05,P<0.01),while HDL-C levels were significantly increased(P<0.05,P<0.01).The formation of aortic plaque and hepatic lipid droplets was significantly inhibited to varying degrees(P<0.05,P<0.01).Alpha and beta diversity analyses indicated good separation in sample clusters of each group.Compared with the control group,the abundance of Akkermansia in the model group significantly decreased(P<0.01).But the treatment with XXK increased the abundance of Akkermansia(P<0.05),which negatively correlated with the levels of TC,TG and LDL-C,and hepatic lipid droplet area(P<0.05,P<0.01).Conclusion The lipid lowering and anti-AS effects of XXK may be related to its modulation of gut microbiota dysbiosis and enhancement of the abundance of the beneficial bacterium Akkermansia.

关 键 词：地奥心血康 降脂 抗动脉粥样硬化 肠道菌群 相关性 小鼠 

分 类 号：R285.5[医药卫生—中药学]