高效液相色谱法同时测定柴葛解酒颗粒中6种有效成分的含量  

Simultaneous Determination of Six Active Components in Chaige Hangover-alleviating Granules by HPLC

作　　者：赵飞雨 陈颖 张荣珍 毕梦非 高慧慧 杨秀秀 汪荣斌[3] 张艳华 王存琴 ZHAO Feiyu;CHEN Ying;ZHANG Rongzhen;BI Mengfei;GAO Huihui;YANG Xiuxiu;WANG Rongbin;ZHANG Yanhua;WANG Cunqin(School of Pharmacy,Wannan Medical College,Wuhu 241002 Anhui,China;Dapartment of Emergency Internal Medicine,Wuhu Hospital of Traditional Chinese Medicine,Wuhu 241000 Anhui,China;Institute of Chinese Medicine Resources,Anhui College of Traditional Chinese Medicine,Wuhu 241002 Anhui,China)

机构地区：[1]皖南医学院药学院,安徽芜湖241002 [2]芜湖市中医医院急诊内科,安徽芜湖241002 [3]安徽中医药高等专科学校中药资源研究所,安徽芜湖241002

出　　处：《中药新药与临床药理》2024年第12期1902-1906,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：安徽省重点研究与开发计划项目(2022e07020067);安徽高校自然科学研究重大项目(2023AH040257,2023AH040268,KJ2021ZD0148);芜湖市科技计划项目(2022cg41,2022jc35)。

摘　　要：目的建立高效液相色谱(HPLC)法同时测定柴葛解酒颗粒中6种有效成分(葛根素、二氢杨梅素、鸢尾苷、葛花苷、槲皮素、柴胡皂苷A)的含量,为其质量标准的完善提供参考。方法采用Agilent 5 HC-C18色谱柱(4.6 mm×250 mm,5μm);以0.2%磷酸水(A)-乙腈(B)为流动相,梯度洗脱;流速:1.0 mL·min^(-1);柱温:30℃;检测波长:210 nm;进样量:10μL。结果在上述色谱条件下,柴葛解酒颗粒中葛根素、二氢杨梅素、鸢尾苷、葛花苷、槲皮素、柴胡皂苷A可实现较好的分离,其线性范围分别为21.16~677.00μg·mL^(-1)(r=1.0000)、11.16~357.00μg·mL^(-1)(r=0.9997)、19.28~617.00μg·mL^(-1)(r=0.9999)、10.38~332.00μg·mL^(-1)(r=0.9999)、15.38~492.00μg·mL^(-1)(r=0.9998)、15.50~496.00μg·mL^(-1)(r=0.9996),各成分均有良好的线性关系;平均加样回收率在98.43%~101.35%之间,RSD均小于3%。结论所建立的方法能同时测定柴葛解酒颗粒中葛根素、二氢杨梅素、鸢尾苷、葛花苷、槲皮素、柴胡皂苷A的含量。该方法准确、重复性好、可操作性强,可为柴葛解酒颗粒的质量评价与控制提供依据。Objective HPLC method was established to simultaneously determine the content of six active components including puerarin,dihydromyricetin,tectoridin,kakkalide,quercetin,and saikosaponin A in Chaige Hangover-alleviating Granules(CAG),and to provide a reference for the improvement of its quality standard.Methods The separation was performed on an Agilent 5HC-C18 column(4.6 mm×250 mm,5μm)with 0.2%phosphoric acid in water(A)-acetonitrile(B)as the mobile phase in a gradient elution at a flow rate of 1.0 mL·min-1.The column temperature was 30℃,the detection wavelength was 210 nm,and the injection volume was 10μL.Results A good separation of puerarin,dihydromyricetin,tectoridin,kakkalide,quercetin,and saikosaponin A in CAG could be achieved under the above chromatographic conditions,with the linear ranges of 21.16~677.00μg·mL^(-1)(r=1.0000),11.16~357.00μg·mL^(-1)(r=0.9997),19.28~617.00μg·mL^(-1)(r=0.9999),10.38~332.00μg·mL^(-1)(r=0.9999),15.38~492.00μg·mL^(-1)(r=0.9998),and 15.50~496.00μg·mL^(-1)(r=0.9996)respectively,showing a good linear correlation.The average recoveries ranged from 98.43%~101.35%,and the RSDs were all less than 3%.Conclusion The method can simultaneously determine the content of puerarin,dihydromyricetin,tectoridin,kakkalide,quercetin,and saikosaponin A in the CAG.Moreover,the established method is accurate,reproducible and operable,which provides the basis for the quality evaluation and control of CAG.

关 键 词：柴葛解酒颗粒 高效液相色谱法 质量标准 含量测定 

分 类 号：R284.1[医药卫生—中药学]