骨蚀再造丸对激素性股骨头坏死大鼠炎症因子的影响  

Effects of Gushi Zaizao Pills on Inflammatory Factors in Rats with Steroid-Induced Osteonecrosis of the Femoral Head

作　　者：沈彩红 邬国松 王建忠[3] 郭永昌 杨志敏 李文茜 张瑾 柴爽 冯小艳 郭家巧 曹玉举 SHEN Cai-Hong;WU Guo-Song;WANG Jian-Zhong;GUO Yong-Chang;YANG Zhi-Min;LI Wen-Xi;ZHANG Jin;CHAI Shuang;FENG Xiao-Yan;GUO Jia-Qiao;CAO Yu-Ju(Henan Provinical Key Laboratory of Osteonecrosis Research of Traditional Chinese Medicine,Henan Provincial Orthopaedic Traditional Chinese Medicine Diagnosis and Treatment Engineering Technology Research Center,Zhengzhou Traditional Chinese Hospital of Orthopaedics,Zhengzhou 450016 Henan,China;Baiyun Branch of Nanfang Hospital,Guangzhou 510080 Guangdong,China;The Second Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010030 Inner Mongolia,China;Henan Provincial Orthopaedic Hospital,Zhengzhou 450016 Henan,China)

机构地区：[1]郑州中医骨伤病医院河南省骨坏死研究中医药重点实验室,河南省骨伤病中医诊疗工程技术研究中心,河南郑州450016 [2]南方医院白云分院,广东广州510080 [3]内蒙古医科大学第二附属医院,内蒙古呼和浩特010030 [4]河南省骨科医院,河南郑州450016

出　　处：《广州中医药大学学报》2024年第12期3252-3258,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金地区科学基金项目(编号:81860401);河南省自然科学基金项目(编号:232300421193);河南省医学科技攻关计划项目(编号:SBGJ202103103);河南省中医药科学研究专项课题(编号:2022ZY2064、2022ZY1182、20-21ZY3023);河南省重点研发与推广专项(科技攻关)项目(编号:232102311202)。

摘　　要：【目的】观察骨蚀再造丸对激素性股骨头坏死的治疗作用及机制。【方法】将雄性SD大鼠随机分为正常组、模型组、仙灵骨葆胶囊组和骨蚀再造丸组。除正常组,其他各组大鼠复制激素性股骨头坏死模型。成功造模后,连续给药6周。取大鼠胸腺和脾脏计算免疫器官指数,酶联免疫吸附法(ELISA)测定大鼠关节液白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)的含量,苏木素-伊红(HE)染色法测定空骨陷窝率、骨小梁面积比,Micro-CT分析股骨头参数[骨体积分数(BV/TV)、骨小梁数量(Tb.N)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp)],小动物骨骼强度测定仪测定股骨头骨骼强度。【结果】与正常组比较,模型组脾脏、胸腺指数显著下降,关节液IL-1β、TNF-α含量显著增加,空骨陷窝率升高,骨小梁面积比降低,BV/TV、Tb.N、Tb.Th降低,Tb.Sp升高,股骨头骨骼强度显著降低,差异均有统计学意义(P<0.05或P<0.01);与模型组比较,仙灵骨葆胶囊组和骨蚀再造丸组脾脏、胸腺指数显著升高,关节液IL-1β、TNF-α含量降低,空骨陷窝率显著降低,骨小梁面积比显著升高,BV/TV、Tb.N、Tb.Th升高,Tb.Sp降低,股骨头骨骼强度显著升高,差异均有统计学意义(P<0.05或P<0.01)。【结论】骨蚀再造丸通过调节细胞免疫,减少炎症因子分泌,进而降低股骨头病理损伤,增强骨骼强度,可有效防止激素性股骨头坏死进一步发展。Objective To observe the therapeutic efficacy and mechanism of Gushi Zaizao Pills for steroid-induced osteonecrosis of the femoral head(SONFH).Methods Male SD rats were randomly divided into normal group,model group,Xianling Gubao Capsules group and Gushi Zaizao Pills group.Except for the normal group,the rats in all other groups were replicated the SONFH model.After successful modeling,the continuous drug administration was carried out for six weeks.The thymus and spleen of the rats were taken to calculate the immune organ indices,the contents of interleukin 1β(IL-1β)and tumor necrosis factorα(TNF-α)in rat synovial fluid were detected by enzyme-linked immunosorbent assay(ELISA),the percentage of empty lacunae and area ratio of trabecular bone was detected by hematoxylin-eosin(HE)staining,and the femoral head parameters[reduction of bone volume fraction(BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th)and trabecular separation(Tb.Sp)]were analyzed by Micro-CT,and bone strength of femoral head was detected by small animal bone strength tester.Results Compared with the normal group,the spleen and thymus indicators were significantly decreased in the model group,the levels of IL-1βand TNF-αin rat synovial fluid were significantly increased,the percentage of empty lacunae was increased,the area ratio of trabecular bone was decreased,the BV/TV,Tb.N and Tb.Th were decreased,the Tb.Sp was increased,the bone strength of femoral head was significantly decreased,the differences being all statistically significant(P<0.05 or P<0.01).Compared with the model group,the spleen and thymus indicators were significantly increased in Xianling Gubao Capsules group and Gushi Zaizao Pills group,the levels of IL-1βand TNF-αin rat synovial fluid were decreased,the percentage of empty lacunae was significantly decreased,the area ratio of trabecular bone was significantly increased,the BV/TV,Tb.N and Tb.Th were increased,the Tb.Sp was decreased,and the bone strength of femoral head was significantly increased,the differenc

关 键 词：骨蚀再造丸 激素性股骨头坏死 脏器指数 炎症因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]