永恒蛋白基因/周期蛋白基因介导多条通路在昼夜节律中的作用  

The role of Timeless/Period gene mediated multiple pathways in circadian rhythm

作　　者：王士杰 文登台 孙国琪 王京峰 高颖晖 Wang Shijie;Wen Dengtai;Sun Guoqi;Wang Jingfeng;Gao Yinghui(School of Physical Education,Ludong University,Yantai 264025,Shandong Province,China)

机构地区：[1]鲁东大学体育学院,山东省烟台市264025

出　　处：《中国组织工程研究》2025年第20期4305-4315,共11页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(32000832),项目名称:心肌salt基因介导运动训练与高盐摄入调控心脏衰老的机制研究,项目负责人:文登台;山东省高等学校青年创新团队发展计划项目(2023RW057),项目名称:脂肪组织Gαq/Gγ1/bmm通路诱导的遗传性肥胖及其并发症对运动适应的研究,项目负责人:文登台。

摘　　要：背景:昼夜节律与大多数哺乳动物及昆虫的生命活动息息相关。永恒蛋白基因作为编码永恒蛋白基因-周期蛋白基因复合物的关键成分在产生昼夜节律过程中起到十分关键的作用,但其在昼夜节律中的作用机制仍不清楚。目的:通过整理永恒蛋白基因、周期蛋白基因、昼夜节律、环境及隐花色素基因之间的关系,更加全面地认识昼夜循环的入核、积累机制及环境对昼夜节律的影响。方法:应用计算机在Web of Science核心合集、PubMed数据库及CNKI中进行检索,以“永恒蛋白基因,周期蛋白基因,昼夜节律,环境”为中文检索词,以“Timeless,Period,circadian rhythm,environment”为英文检索词,通过全文阅读逐步排除非相关文献,最终纳入126篇文献进行综述。结果与结论:在昼夜时钟中,昼夜自发输出周期蛋白kaput和CYCLE激活永恒蛋白基因/周期蛋白基因,永恒蛋白基因调控周期蛋白基因的入核机制及稳定性,而周期蛋白基因也可以通过一些机制单独入核。酪蛋白激酶2、Shaggy蛋白激酶及双倍时间基因都可以通过磷酸化永恒蛋白基因/周期蛋白基因的方式来调节昼夜节律,参与转录。隐花色素基因介导的永恒蛋白基因降解对转录的完整性有十分重要的作用。环境因素、膳食方式等外界因素均可以通过永恒蛋白基因/周期蛋白基因对昼夜节律产生影响,而限时进食可作为一种改善昼夜节律紊乱的有效方法。BACKGROUND:Circadian rhythms are closely related to the life activities of most mammals and insects.Timless gene plays a crucial role in the generation of circadian rhythms as key components encoding the Timeless/Period gene complex.However,its specific mechanism in circadian rhythms is still unclear.OBJECTIVE:To study the relationship between Timless protein gene,Period gene,circadian rhythm,environment and cryptochrome gene,so as to have a more comprehensive understanding of the nucleation and accumulation mechanism of circadian cycle and the influence of environment on circadian rhythm.METHODS:Literature retrieval was conducted in the Web of science Core Collection database,PubMed and CNKI,and the relevant literature was searched,consulted and screened after the keyword was set as“Timless,Period,circadian rhythm,environment”in English and Chinese.Non-relevant literature was progressively excluded through full-text reading,and 126 papers were finally included for the review.RESULTS AND CONCLUSION:In the circadian clock,the circadian spontaneous output of the cyclins kaput and CYCLE activate the Timeless/Period gene,Timeless gene regulates the nucleation mechanism and stability of Period gene,and Period gene can also be nucleated individually by a number of mechanisms.Casein kinase 2,Shaggy protein kinase and double time genes can regulate circadian rhythms and participate in transcription by phosphorylating Timeless gene/Period gene.Cryptochrome gene-mediated degradation of Timeless gene has a very important role in transcriptional integrity.External factors such as environmental factors and dietary patterns can influence circadian rhythms through the Timeless gene/Period gene.Interestingly,time-restricted eating can be used as an effective way to improve circadian rhythm disturbances.

关 键 词：昼夜节律 永恒蛋白基因 环境 隐花色素 周期蛋白基因 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] G804.2[文化科学—运动人体科学]