圆锥乌头中二萜生物碱类成分及其化学分类学研究  

Diterpenoid alkaloids with chemotaxonomic significance from Aconitum paniculigerum

作　　者：欧彦希 李梅 梁文成 马嘉仪 文婕 王金秋 朱晨莹 朱凌捷 陈琳[1,3] 周先礼[1,2] OU Yanxi;LI Mei;LIANG Wencheng;MA Jiayi;WEN Jie;WANG Jinqiu;ZHU Chenying;ZHU Lingjie;CHEN Lin;ZHOU Xianli(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;Affiliated Hospital of Southwest Jiaotong University&The Third People Hospital of Chengdu,Chengdu 610031,China;Yibin Research Institute,Southwest Jiaotong University,Yibin 644000,China)

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031 [2]西南交通大学附属医院成都市第三人民医院,四川成都610031 [3]西南交通大学宜宾研究院,四川宜宾644000

出　　处：《中草药》2024年第22期7591-7600,共10页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(82073734);宜宾双城市校协议专项研究基金(YBSCXY2023020005);中央高校基本科研业务费专项资金(2682023ZTPY051)。

摘　　要：目的 对圆锥乌头Aconitum paniculigerum中二萜生物碱化学成分及其化学分类学意义进行研究,以促进该植物物种的鉴定与分类。方法 采用正相硅胶柱色谱对圆锥乌头中总生物碱进行分离纯化,并通过高分辨率电喷雾质谱、1D NMR和2DNMR等谱学技术鉴定化合物的结构。同时,通过网络分析探讨这些化合物的化学分类学意义。结果 从圆锥乌头块根中分离得到15个二萜生物碱和1个异喹啉生物碱,分别鉴定为圆锥乌碱甲(1)、次乌头碱(2)、黄草乌碱丁(3)、塔拉萨敏(4)、乌头碱(5)、尼奥灵(6)、beiwudine(7)、12-epi-acetyldehydronapelline(8)、12-epi-19-dehydrolucidusculine(9)、15-acetylsongoramine(10)、12-epi-15-acetylnapelline(11)、12-表欧乌头碱(12)、宋果灵(13)、yesoxine(14)、中乌头碱(15)、海罂粟碱(16)。结论 化合物1为新天然产物,其余化合物均为首次从该植物中分离得到。其中化合物7在乌头属植物中分布有限,可作为区分圆锥乌头与其他相似物种的潜在化学分类标记,具有一定的化学分类学价值。Objection To study the diterpenoid alkaloids and their chemotaxonomic significance in Aconitum paniculigerum,in order to facilitate the identification and classification of this plant species.Methods Total alkaloids from A.paniculigerum were separated and purified using silica gel column chromatography.The structures of the isolated compounds were identified by HR-ESI-MS,1D NMR and 2D NMR spectroscopic techniques.Additionally,the chemotaxonomic significance of these compounds was explored through network analysis.Results A total of fifteen diterpenoid alkaloids and one isoquinoline alkaloid were isolated from A.paniculigerum and identified as paniculine A(1),hypaconitine(2),sachaconitine(3),talatisamine(4),aconitine(5),neoline(6),beiwudine(7),12-epi-acetyldehydronapelline(8),12-epi-19-dehydrolucidusculine(9),15-acetylsongoramine(10),12-epi-15-acetylnapelline(11),12-epi-napelline(12),songorine(13),yesoxine(14),mesaconitine(15)and glaucine(16).Conclusion Compound 1 was a new natural product,and the other compounds were isolated from this plant for the first time.Among them,compound 7 had a limited distribution within the Aconitum genus and can serve as a potential chemotaxonomic marker for distinguishing A.paniculigerum from other similar species,demonstrating significant chemotaxonomic value.

关 键 词：乌头属 圆锥乌头 二萜生物碱 网络分析 化学分类学 圆锥乌碱甲 次乌头碱 黄草乌碱丁 beiwudine 

分 类 号：R284.1[医药卫生—中药学]