鸡TRIM27.2基因的生物信息学分析及其抗传染性法氏囊病病毒的功能初探  

Bioinformatics Analysis of Chicken TRIM27.2 Gene and Its Function of Anti-infectious Bursal Disease Virus

作　　者：尹娜 吴双 王佳兴 平玉宇 黄煊 白皓 陈世豪[1,2] 常国斌 YIN Na;WU Shuang;WANG Jiaxing;PING Yuyu;HUANG Xuan;BAI Hao;CHEN Shihao;CHANG Guobin(College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009;College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009;Joint Laboratory of International Cooperation on Agriculture and Agricultural Product Safety,Ministry of Education,Institute of Agricultural Science and Technology Development,Yangzhou University,Yangzhou,Jiangsu 225009)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]扬州大学兽医学院,江苏扬州225009 [3]扬州大学农业科技发展研究院,教育部农业与农产品安全国际合作联合实验室,江苏扬州225009

出　　处：《中国家禽》2024年第12期7-14,共8页China Poultry

基　　金：国家肉鸡产业技术体系(CARS-41)。

摘　　要：试验旨在检测鸡TRIM27.2基因在不同组织中的表达,并探究其在鸡巨噬细胞系HD11细胞中的抗病毒作用。试验对鸡TRIM27.2基因进行克隆、生物信息学分析,利用实时荧光定量、共聚焦显微镜技术以及蛋白质免疫印迹等方法对其功能进行初步分析。结果显示:鸡TRIM27.2基因CDS区长1431bp,编码476个氨基酸,属于不稳定、亲水性蛋白,无跨膜结构和信号肽,有35个潜在磷酸化位点和18个泛素化位点;二级结构主要由α-螺旋、β-转角、延伸链及无规则卷曲组成;在三级结构上,鸡TRIM27.2与人TRIM27相似;TRIM27.2基因在鸡多组织中均有表达,心脏中的表达量最高;鸡TRIM27.2蛋白显著抑制传染性法氏囊病病毒(IBDV)复制。研究表明TRIM27.2蛋白具有抑制IBDV复制的作用,试验为进一步揭示鸡TRIM27.2的生物学功能奠定基础。The aim of the experiment was to detect the expression of chicken TRIM27.2 gene in different tissues and to investigate its antiviral effects in chicken macrophage cell line HD11 cells.The chicken TRIM27.2 gene was cloned,bioinformatically analysed,and its function was preliminarily analysed using real-time fluorescence quantification,confocal microscopy technique and protein immunoblotting.The results showed that the CDS region of chicken TRIM27.2 gene was 1431 bp,encoding 476 amino acids,which belonged to unstable and hydrophilic protein without transmembrane structure and signal peptide,with 35 potential phosphorylation sites and 18 ubiquitination sites.The secondary structure mainly consisted ofα-helix,β-turns,extended strand,and irregular coiling.In terms of tertiary structure,chicken TRIM27.2 was similar to human in the tertiary structure,chicken TRIM27.2 was similar to human TRIM27;TRIM27.2 gene was expressed in multiple tissues of chickens,with the highest expression in the heart;Chicken TRIM27.2 protein significantly inhibited the replication of infectious bursal disease virus(IBDV).The results indicated that TRIM27.2 protein inhibited the replication of IBDV.This study laid the foundation for further revealing the biological functions of chicken TRIM27.2.

关 键 词：鸡 TRIM27.2基因 生物信息学 组织表达 传染性法氏囊病病毒 

分 类 号：S831.2[农业科学—畜牧学]