也迷离鸡线粒体全基因组序列特征及系统发育分析  

Characterization of Complete Mitochondrial Genome Sequence of Yemili Chicken(Gallus gallus)and Phylogenetic Analysis

作　　者：许赫 李甜甜 张洁[1] 李莹莹 朱荷青 梁千千 董华娇 荣旭冉 杨素荣 赵全庄 韩吉龙 杨敏 XU He;LI Tiantian;ZHANG Jie;LI Yingying;ZHU Heqing;LIANG Qianqian;DONG Huajiao;RONG Xuran;YANG Surong;ZHAO Quanzhuang;HAN Jilong;YANG Min(College of Animal Science and Technology,Shihezi University,Shihezi,Xinjiang 832000;National Yili Goose Conservation Farm,Emin County Hengxin Industrial Co.,Ltd.,Tarbagatay Prefecture,Tacheng,Xinjiang 834600)

机构地区：[1]石河子大学动物科技学院,新疆石河子832000 [2]额敏县恒鑫实业有限公司国家伊犁鹅保种场,新疆塔城834600

出　　处：《中国家禽》2024年第12期15-23,共9页China Poultry

基　　金：国家自然科学基金项目(32060745);石河子大学育种专项(YZZX202005);石河子大学青年创新拔尖项目(CXBJ201903)。

摘　　要：为揭示也迷离鸡线粒体全基因组特征,并探讨也迷离鸡起源驯化,研究利用Illu‐mina Nova Seq 6000二代测序技术,获得完整的也迷离鸡全基因组测序信息,将原始数据进行质控过滤,并经MitoZ v3.4软件组装注释成完整的线粒体基因组。从EBI数据库下载37个国内外不同品种鸡及鹌鹑的线粒体全基因组DNA序列,基于Mega-X软件邻接法(Neighbor-Joining,NJ)构建系统发育树。结果显示:也迷离鸡线粒体基因组全序列长度为16790 bp,包括13个蛋白编码基因、22个tRNA基因、2个rRNA基因和1个非编码控制区(D-loop区)。线粒体基因组中核苷酸比例为T(23.70%)、C(32.50%)、A(30.30%)、G(13.50%),AT-skew>0,GC-skew<0。系统发育分析显示,也迷离鸡与红原鸡及来航鸡的亲缘关系较近。结果表明,也迷离鸡在驯化过程中可能导入原鸡血液,存在来航鸡等商业鸡的基因渗入。In order to reveal the whole mitochondrial genome characteristics of Yemili chicken and explore the origin and domestication history of Yemili chicken,resequencing was performed by using Illumina Nova Seq 6000,then filtered by quality control and assembled into a complete mitochondrial genome using MitoZ v3.4 software.The mitochondrial genome DNA sequences of 37 chicken breeds and one quail were downloaded from European Institute of Bioinformatics,and the phylogenetic tree was constructed by Neighbor-Joining(NJ)method using Mega-X software.The results showed that the complete mitochondrial genome of Yemili chicken was 16790 bp in length,including 13 protein-coding genes,22 tRNA genes,2 rRNA genes and 1 noncoding control region(D-loop region).The proportion of nucleotides in the mitochondrial genome was T(23.70%),C(32.50%),A(30.30%),G(13.50%),AT-skew>0,GC-skew<0.The results of phylogenetic analysis showed Yemili chicken had a close genetic relationship with the red junglefowl and leghorn chicken.The results indicated that Yemili chicken might import the red junglefowl blood during domestication,and had the gene infiltration of commercial chickens such as Leghorn chicken.

关 键 词：也迷离鸡 线粒体基因组 序列特征 系统发育 

分 类 号：S831.2[农业科学—畜牧学]