Translocation of ssDNA through Charged Graphene Nanopores:Effect of the Charge Density  

作　　者：Yuan-Shuo Zhang Zhi-Ya Qi Ming-Ming Ding Ming-Lun Li Tong-Fei Shi 

机构地区：[1]School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou,510006,China [2]Jieyang Branch of Chemistry and Chemical Engineering Guangdong Laboratory,Jieyang,515200,China [3]Department of Polymer Science and Engineering,University of Massachusetts Amherst,120 Governors Drive,Amherst,Massachusetts,01003,USA

出　　处：《Chinese Journal of Polymer Science》2024年第12期2048-2058,I0013,共12页高分子科学（英文版）

基　　金：This work was financially supported by the National Natural Science Foundation of China(Nos.22373025 and 22227804).

摘　　要：Nanopore sequencing harnesses changes in ionic current as nucleotides traverse a nanopore,enabling real-time decoding of DNA/RNA sequences.The instruments for the dynamic behavior of substances in the nanopore on the molecular scale are still very limited experimentally.This study employs all-atom molecular dynamics(MD)simulations to explore the impact of charge densities on graphene nanopore in the translocation of single-stranded DNA(ssDNA).We find that the magnitude of graphene’s charge,rather than the charge disparity between ssDNA and graphene,significantly influences ssDNA adsorption and translocation speed.Specifically,high negative charge densities on graphene nanopores are shown to substantially slow down ssDNA translocation,highlighting the importance of hydrodynamic effects and electrostatic repulsions.This indicates translocation is crucial for achieving distinct ionic current blockades,which plays a central role for DNA sequencing accuracy.Our findings suggest that negatively charged graphene nanopores hold considerable potential for optimizing DNA sequencing,marking a critical advancement in this field.

关 键 词：Nanopore sequencing Graphene nanoslit DNA translocation Electroosmotic flow 

分 类 号：TQ127.11[化学工程—无机化工] TB383.1[一般工业技术—材料科学与工程]