茯苓中一个新孕甾烷类化合物  

A new pregnane steroid from Poria cocos

作　　者：肖超远 郝志友[1,2] 杨梦 周诗琪[1,2] 张俊阳 郑晓珂 孙彦君 冯卫生[1,2,3] XIAO Chao-yuan;HAO Zhi-you;YANG Meng;ZHOU Shi-qi;ZHANG Jun-yang;ZHENG Xiao-ke;SUN Yan-jun;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Research Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China;Co-construction of Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan&Education Ministry,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046 [3]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《药学学报》2024年第12期3325-3329,共5页Acta Pharmaceutica Sinica

基　　金：中央引导地方科技发展专项资金([2016]149);河南省高层次人才特殊支持计划(ZYQR201810080)

摘　　要：采用ODS、MCI gel CHP20以及硅胶等柱色谱技术并结合半制备液相对茯苓95%乙醇提物进行分离纯化,运用NMR、MS、IR、计算NMR等方法,对分离得到的化合物进行结构鉴定。从茯苓中共分离得到7个化合物,分别鉴定为20S-2β,3α,15α,19,20-羟基-孕甾-7-烯(1)、去氢齿孔酸乙酰酯(2)、齿孔酸乙酰酯(3)、去氢齿孔酸(4)、齿孔酸(5)、去氢茯苓酸(6)、茯苓酸(7)。化合物1为新孕甾烷类化合物。The 95% ethanol extract of Poria cocos was separated and purified by ODS,MCI gel CHP20 and silica gel column chromatography combined with the semi-preparative HPLC.The chemical structures of the isolated compounds were identified by NMR,MS,IR,and calculated NMR methods.Seven compounds were isolated from Poria cocos and identified as 20S-2β,3α,15α,19,20-hydroxy-pregnane-7-ene(1),dehydroeburicoic acid monoacetate(2),eburicoic acid acetate(3),dehydroeburicoic acid(4),eburicoic acid(5),dehydropachymic acid(6),pachymic acid(7).Compound 1 is a new pregnane steroid.

关 键 词：茯苓 化学成分 孕甾烷 四环三萜 结构鉴定 

分 类 号：R284[医药卫生—中药学]