穿心草全草中两个新的二苯醚类化合物  

Two new diphenyl ethers from the whole plant of Canscora lucidissima

作　　者：张梦雨 刘娇 刘畅 张涛[2] 邹忠梅 ZHANG Meng-yu;LIU Jiao;LIU Chang;ZHANG Tao;ZOU Zhong-mei(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China;School of Traditional Chinese Materia Medica,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]中国医学科学院北京协和医学院,药用植物研究所,北京100193 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《药学学报》2024年第12期3335-3341,共7页Acta Pharmaceutica Sinica

基　　金：中国医学科学院医学与健康科技创新工程项目(2022-I2M-1-017);国家“重大新药创制”科技重大专项(2019ZX09735002)

摘　　要：采用硅胶柱色谱、MCI柱色谱、聚酰胺柱色谱、Sephadex LH-20柱色谱及ODS柱色谱,结合半制备高效液相色谱等技术手段,从穿心草(Canscora lucidissima)全草95%乙醇提取物中分离纯化得到12个化合物。依据其理化性质和波谱数据(UV、IR、MS、NMR)等对所分离得到的化合物进行结构鉴定,分别为穿心草二苯醚A (1)、穿心草二苯醚B (2)、穿心草二苯甲酮A (3)、(-)-松脂素(4)、间羟基苯甲酸(5)、ω-羟基大黄素(6)、二氢山柰酚(7)、6,7-二羟基香豆素(8)、4-羟基-3,5-二甲氧基苯甲酸(9)、4-羟基-3,5-二甲氧基肉桂酸(10)、2-羟基-3-甲氧基苯甲酸(11)、(-)-丁香树脂酚(12)。其中,化合物1和2为新化合物,化合物3为新天然产物。化合物8通过抑制脂多糖诱导的RAW264.7细胞NO的生成,表现出一定的抗炎活性。Twelve compounds were isolated and purified from 95% ethanol extract of the whole plant of C.lucidissima by silica gel column chromatography,MCI column chromatography and ODS column chromatography,polyamide column chromatography,Sephadex LH-20 column chromatography,combined with semi-preparative HPLC methods.Based on the physicochemical properties and spectral data(UV,IR,MS,NMR),the structures of the isolates were identified as canlucdiphenyl ether A(1),canlucdiphenyl ether B(2),canlucbenzophenone A(3),(-)-pinoresinol(4),3-hydroxybenzoic acid(5),1,3,8-trihydroxy-6-hydroxymethylanthraquinone(6),dihydrokaempferol(7),6,7-dihydroxycoumarin(8),4-hydroxy-3,5-dimethoxybenzoic acid(9),4-hydroxy-3,5-dimethoxycinnamic acid(10),2-hydroxy-3-methoxybenzoic acid(11),(-)-syringaresinol(12).Among them,compounds 1 and 2 are two new diphenyl ether and 3 is a new natural product.Compound 8 exhibited some antiinflammatory activity by inhibiting the lipopolysaccharide-induced NO production in RAW264.7 cells.

关 键 词：龙胆科 穿心草 化学成分 二苯醚 抗炎作用 

分 类 号：R284[医药卫生—中药学]