千层塔中一个新的生物碱及其生物活性研究  

A new alkaloid from Huperzia serrata and its biological activity

作　　者：乔晓婷 成欣欣 王丹[1] 来美含 王嘉琪 王晓敏 阮彩莲[2] 曹朵 QIAO Xiao-ting;CHENG Xin-xin;WANG Dan;LAI Mei-han;WANG Jia-qi;WANG Xiao-min;RUAN Cai-lian;CAO Duo(Shaanxi Key Laboratory of Research and Utilization of Resource Plants on the Loess Plateau,Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources,College of Life Sciences,Yan'an University,Yan'an 716000,China;Medical School of Yan'an University,Yan'an 716000,China)

机构地区：[1]延安大学生命科学学院,陕西省黄土高原资源植物研究与利用省市共建重点实验室,陕西省区域生物资源保育与利用工程技术研究中心,陕西延安716000 [2]延安大学医学院,陕西延安716000

出　　处：《药学学报》2024年第12期3342-3346,共5页Acta Pharmaceutica Sinica

基　　金：陕西省自然科学基金(2024JC-YBQN-0920);陕西省科协技术学会青年人才托举计划项目(20220321)

摘　　要：采用ODS反相硅胶、AB-8大孔吸附树脂、葡聚糖凝胶Sephadex LH-20、氧化铝柱层析和半制备液相制备色谱等方法,对千层塔甲醇提取物进行较为系统的分离纯化,通过MS、IR、NMR等方法,对分离到的化合物进行结构鉴定。从千层塔中共分离鉴定了4个生物碱化合物,分别为serratinine C (1),lycobeline C (2),diphaladin A (3)和crenatine (4)。其中,化合物1为新的生物碱类化合物,化合物2~4是首次从千层塔中分离。体外生物活性实验表明,化合物1能够明显降低脂多糖(lipopolysaccharide,LPS)诱导的神经胶质细胞中一氧化氮(nitric oxide,NO)和活性氧(reactive oxygen species,ROS)水平,具有显著抗氧化生物活性。The methanol extract of Huperzia serrata was separated and purified by ODS,AB-8 macroporous adsorption resin,dextran gel Sephadex LH-20 and silica gel column chromatography combined with the semi-pre HPLC.The chemical structures of the isolated compounds were identified by MS,IR,NMR,etc.Four compounds were isolated from Huperzia serrata and identified,named as serratinine C(1),lycobeline C(2),diphaladin A(3)and crenatine(4).Compound 1 is a new alkaloid,compounds 2-4 were isolated for the first time.In vitro biological activity experiments showed that compound 1 could significantly reduce the levels of nitric oxide and reactive oxygen species in glial cells induced by lipopolysaccharide and had significant antioxidant biological activity.

关 键 词：千层塔 化学成分 生物碱 结构鉴定 抗氧化 

分 类 号：R284[医药卫生—中药学]