兰州市<5岁儿童病毒性腹泻病原谱分析  

Pathogen spectrum analysis of viral diarrhea in children under 5 years old in Lanzhou City

作　　者：张连梅 王宇红[1,2] 毛雪 刘璐 杜佳铭 ZHANG Lianmei;WANG Yuhong;MAO Xue;LIU Lu;DU Jiaming(Lanzhou Municipal Center for Disease Control and Prevention,Lanzhou,Gansu 730030,China;College of Environment and Resources,Northwest Normal University)

机构地区：[1]兰州市疾病预防控制中心,甘肃兰州730030 [2]西北师范大学环境与资源学院

出　　处：《现代疾病预防控制》2024年第12期931-934,共4页MODERN DISEASE CONTROL AND PREVENTION

基　　金：兰州市卫生健康科技发展项目(2021025)。

摘　　要：目的了解兰州市<5岁儿童病毒性腹泻的主要病原体,为疾病防控提供科学依据。方法2022—2023年在兰州大学第二医院和兰州市第一人民医院采集<5岁住院及门诊腹泻患儿发病7 d内的粪便标本,采用实时荧光聚合酶链式反应(PCR)方法检测轮状病毒、诺如病毒、腺病毒、星状病毒和札如病毒感染情况,计数资料组间比较运用χ^(2)检验,分析病毒阳性情况。结果320份腹泻儿童粪便标本共检出病毒阳性144份,阳性率为45.00%,其中轮状病毒阳性46份,阳性率为14.38%,均为轮状病毒A型;诺如病毒阳性62份,阳性率为19.38%,其中93.55%为诺如病毒G2型;腺病毒35份,阳性率为10.94%;星状病毒58份,阳性率为18.13%;札如病毒34份,阳性率为10.63%。多重感染59份,阳性率高达18.44%,其中二重感染33份,阳性率为10.31%,以诺如病毒-星状病毒合并感染为主;三重感染22份,阳性率为6.88%,以轮状病毒-星状病毒-诺如病毒合并感染为主;四重和五重感染各2份。不同年龄组腹泻儿童轮状病毒、腺病毒和总阳性率差异均有统计学意义(χ^(2)值分别为12.072、10.182、9.738,P均<0.05),而诺如病毒、星状病毒、札如病毒及多重感染阳性率差异均无统计学意义(χ^(2)值分别为5.039、5.129、5.589、5.433,P均>0.05),腺病毒阳性率以<1岁年龄组(19.54%)最高,轮状病毒和总阳性率以1~<2岁年龄组最高(分别为22.13%、53.28%)。结论兰州市5岁以下腹泻儿童病毒感染以1~<2岁年龄组为主,主要病原体为诺如病毒和星状病毒,多重感染情况较为严峻,应加强健康教育宣传。Objective To understand the main pathogens of viral diarrhea in children under 5 years old in Lanzhou City,so as to provide scientific evidence for disease prevention and control.Methods Stool samples from inpatients and outpatients of children<5 years old with diarrhea were collected within 7 days of onset of illness at the Second Hospital of Lanzhou University and the First People's Hospital of Lanzhou City from 2022 to 2023.Real-time fluorescent PCR was used to detect rotavirus,norovirus,adenovirus,astrovirus and zaruvirus.The comparison between groups for count data was conducted using Chi-square test;the status of virus positivity was analyzed.Results A total of 144 virus positive samples were detected in 320 stool samples of diarrhea children,with the positive rate of 45.00%.There were 46 positive samples for rotavirus,and with the positive rate of14.38%,and all of which were rotavirus type A;62 samples were positive for norovirus,with the positive rate of 19.38%,of which93.55%were norovirus G2 type;35 samples were positive for adenovirus,and the positive rate was 10.94%;58 samples were positive for astrovirus,with the positive rate of 18.13%;34 samples were positive for zaruvirus,and the positive rate was 0.63%;59 samples were positive for multiple infection,with the positive rate of 18.44%.There were 33 positive samples for double infection,and the positive rate was 10.31%.The co-infection of norovirus and astrovirus was predominant.There were 22samples were positive for triple infection,with the positive rate of 6.88%,and the co-infection of rotavirus,astrovirus and norovirus was the main infection;Two samples were positive for quadruple infection,and two for quintuple infection.The positive rates of rotavirus and adenovirus,and the overall positive rate in children with diarrhea among different ages were statistically significant(χ^(2)values were 12.072,10.182 and 9.738,respectively,and all P<0.05),while there was no statistical significance in the positive rates of norovirus,astrovirus,zaruvirus and m

关 键 词：病毒性腹泻 轮状病毒 诺如病毒 星状病毒 儿童 

分 类 号：R725.7[医药卫生—儿科]