异源表达葡萄VvMYB103提高拟南芥盐胁迫耐受能力  

Heterologous overexpression of grape VvMYB103 enhances the salt tolerance of Arabidopsis

作　　者：宗全颖 车永梅 吕晓元[1,2] 叶青 侯丽霞 刘新[1,2] ZONG Quanying;CHE Yongmei;U Xiaoyuan;YE Qing;HOU Lixia;LIU Xin(Shandong University Key Laboratory of Plant Biotechnology,Qingdao,Shandong 266109,China;College of Life Sciences,Qingdao Agricultural University,Qingdao,Shandong 266109,China)

机构地区：[1]山东省高校植物生物技术重点实验室,山东青岛266109 [2]青岛农业大学生命科学学院,山东青岛266109

出　　处：《植物生理学报》2024年第11期1665-1673,共9页Plant Physiology Journal

基　　金：山东省重点研发计划项目(2022LZGCQY019);山东省自然科学基金项目(ZR2023MC074);青岛市自然科学基金项目(23-2-1-178-zyyd-jch)。

摘　　要：葡萄是重要的经济果树,多数栽培品种对盐胁迫敏感,MYB转录因子在抗逆相关基因表达中发挥重要调节作用。本文以耐盐葡萄品种‘左优红’为材料,克隆一个响应盐胁迫的MYB转录因子基因VvMYB103,并对其进行生物信息学分析和表达特性分析,同时以转基因拟南芥为材料研究其与植株耐盐性的关系。结果显示,VvMYB103 cDNA全长为987 bp,编码328个氨基酸;VvMYB103在葡萄根、茎、叶和芽等组织中均有表达,芽中表达量最高。VvMYB103受盐胁迫诱导,并且抗性葡萄品种中的表达量显著高于敏感品种。在盐胁迫下,过表达VvMYB103后拟南芥种子的萌发率提高,过表达VvMYB103株系的生长明显优于野生型,植株相对含水量、叶绿素含量以及超氧化物歧化酶等保护酶活性均高于野生型植株,细胞膜相对透性、丙二醛和过氧化氢含量则低于野生型。这表明,VvMYB103通过维持植株水分平衡和提高植株抗氧化能力,对植株耐盐性起正调节作用。Grape(Vitis vinifera)is an economically significant fruit plant,many cultivated grape varieties are sensitive to salt stress.MYB transcription factors play critical role in the expression of stress related genes.We have isolated a MYB transcription factor gene VvMYB103 from the grape variety'Zuoyouhong'.This paper analyzed the bioinformatic and expression characteristics of VvMYB103.Using transgenic Arabidopsis plants,the function of VvMYB103 in salt tolerance was studied.The results showed,VvMYB103 cDNA is 987 bp in length,encoding 328 amino acids.VvMYB103 was expressed in the roots,stems,leaves,and buds of grapes,with the highest expression level in the buds.VvMYB103 was induced by salt stress,with significantly higher expression levels in salt-resistant grape varieties compared to sensitive ones.Under salt stress,overexpression of VvMYB103 enhanced the germination of transgenic Arabidopsis seeds.The growth of VvMYB103 overexpressing lines was better than that of wild-type plants.VvMYB103 overexpressing lines showed higher relative water content and chlorophyll content,as well as increased antioxidant enzyme activities,such as soD activity.However,they exhibited lower relative membrane permeability,malondialdehyde and hydrogen peroxide content.These findings indicate that VvMYB103 plays a positive role in plant salt tolerance by maintaining plant water balance and improving antioxidant capacity.

关 键 词：VvMYB103 盐胁迫 转录因子 抗氧化 葡萄 

分 类 号：S663.1[农业科学—果树学]