基于不同时间点体温监测探索温度对大鼠出现恶心、呕吐的影响  

The Effects of Temperature on Nausea and Vomiting in Rats were Investigated Based on Temperature Monitoring at Different Time Points

作　　者：王秀岩 崔红蕾 关春雨 陶玲玲 WANG Xiuyan;CUI Honglei;GUAN Chunyu;TAO Lingling(Department of Anesthesiology,Affiliated HongQi Hospital of Mudanjiang Medical University,Mudanjiang 157011,Heilongjiang,China;The Center for Gastrointestinal Endoscopy,Affiliated HongQi Hospital of Mudanjiang Medical University,Mudanjiang 157011,Heilongjiang,China)

机构地区：[1]牡丹江医科大学附属红旗医院麻醉科,黑龙江牡丹江157011 [2]牡丹江医科大学附属红旗医院消化内镜中心,黑龙江牡丹江157011

出　　处：《系统医学》2024年第22期41-44,共4页Systems Medicine

基　　金：牡丹江市科学技术局(HT2022NS070)。

摘　　要：目的研究不同温度对围术期大鼠恶心呕吐的影响及机制研究。方法选取2023年1月牡丹江医科大学比较医学中心提供的雄性大鼠30只,按随机数字表法分为3组,3组均给大鼠腹腔注射顺铂3 mg/kg,造成异食癖模型,将形成异食癖的大鼠分为室温为26℃,湿度为40%~60%的A组,室温为23℃,湿度为40%~60%的B组,室温为18℃,湿度为40%~60%的C组;分别在15 min(T1)、2 h(T2)、4 h(T3)、6 h(T4)、24 h(T5)测大鼠肛温,并采集静脉血,分离血浆测试精氨酸加压素(arginine vasopressin,AVP)含量和皮质醇浓度。结果A组、B组、C组大鼠肛温在T2时分别为(36.82±0.08)℃、(36.59±0.12)℃、(36.22±0.13)℃,差异有统计学意义(F=72.554,P<0.05),3组在T3、T4及T5时比较,差异有统计学意义(P均<0.05);B组血浆中AVP的含量在T3时低于A组,但在T5时又升高,而C组血浆中AVP的含量在T2和T4时升高,差异有统计学意义(P均<0.05);B组在T4和T5时皮质醇浓度升高,C组在T3时皮质醇浓度先下降,到T4时皮质醇浓度开始升高,一直持续到T5,差异有统计学意义(P均<0.05)。组内对比,发现A组内大鼠肛温、AVP含量和皮质醇浓度变化最小。结论室温为26℃,湿度为40%~60%的空间内,大鼠的体温更加趋于稳定,而血浆AVP含量和皮质醇浓度变化最小,发生恶心呕吐的概率是最低的。Objective To study the effect and mechanism of different temperature on nausea and vomiting in perioperative rats.Methods Thirty male rats purchased from the Comparative Medicine Center of Mudanjiang Medical University in January 2023 were selected and divided into three groups according to the random number table metho,and intraperitoneally injected with cisplatin 3 mg/kg to establish the model of pica.The rats with pica were placed into three groups:room temperature 26℃,humidity 40%-60%(A group);room temperature 23℃,humidity 40%-60%(B group);room temperature 18℃,humidity 40%-60%(C group);at 15 min(T1),2h(T2),4 h(T3),6 h(T4),24 h(T5),the anal temperature,blood pressure,blood gas analysis were measured,and venous blood was collected to isolate plasma for testing the content of arginine vasopressin(AVP)and cortisol concentration.Results The rectal temperatures of rats in Groups A,B and C at T2 time point were(36.82±0.08)℃,(36.59±0.12)℃,and(36.22±0.13)℃,respec tively,the difference was statistically significant(F=72.554,P<0.05).There were also statistically significant differences at T3,T4,and T5 time points,in three groups(all P<0.05);The AVP content in group B exhibited a decrease compared to that in group A at T3 time points,followed by a substantial increase at T5 time points.Meanwhile,the plasma AVP content in group C showed a notable increase at T2 and T4 time points,the differences were statistically significant(all P<0.05);The cortisol concentration in Group B increased at time points T4 and T5,while in Group C,it initially decreased at time point T3,then increased at time point T4 and continued until time point T5,the differences were statistically significant(all P<0.05).Minimal changes in anal temperature,AVP content,and cortisol concentration were observed in group A within group comparison.Conclusion In the room temperature of 26℃ and humidity of 40%-60%,the body temperature of rats tends to be more stable,and the changes of plasma AVP content and cortisol concentration are minimal,and

关 键 词：恶心呕吐 大鼠 体温监测 AVP 皮质醇 

分 类 号：R4[医药卫生—临床医学]