高效液相色谱-串联质谱法分析不同产地黄芪化学成分  

Determination of the Differential Compounds in Astragali Radix from Different Origins by High Performance Liquid Chromatography-Mass Spectrometry

作　　者：庄玥 黄艳婷[2] 吴凤丹 李火云 邱蕴绮[2] Zhuang Yue;Huang Yanting;Wu Fengdan;Li Huoyun;Qiu Yunqi(Guangdong University of Technology,Guangzhou 510006,China;Guangdong Institute for Drug Control,Guangzhou 510663,China)

机构地区：[1]广东工业大学,广东广州510006 [2]广东省药品检验所,广东广州510663

出　　处：《广东化工》2024年第22期148-151,共4页Guangdong Chemical Industry

基　　金：广东省医学科学技术研究基金项目(B2021409);广东省中医药局科研项目(20221055)。

摘　　要：建立了高效液相色谱-串联质谱同时测定黄芪中10种化学成分含量的方法,分析了不同产地黄芪中化学成分的差异。采用Agilent Poroshell 120 EC-C_(18)色谱柱(2.1 mm×150 mm,2.7μm)分离,以0.1%甲酸乙腈和0.1%甲酸水溶液作为流动相,0.3 mL/min梯度洗脱,在电喷雾正和负离子模式(ESI±),进行多反应监测(MRM)模式检测。结果显示,10种目标检测成分在各自线性范围内均呈现良好的线性关系,相关系数均大于0.9970。平均加标回收率为94.6%~106.2%,相对标准偏差(RSD)均小于5%。对24批黄芪样品进行测定,发现5批黄芪样品不符合规定的含量要求。山西产地黄芪中总皂苷含量最高,吉林产地黄芪中总黄酮含量最高。通过对总皂苷与总黄酮的含量和比率进行分析,表明产地会影响黄芪中总黄酮和总皂苷的含量。To develop a high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the simultaneous qualitative and quantitative analysis of 10 chemical constituents of Astragali Radix from different origins.The target analytes were separated on an Agilent Poroshell 120 EC-C_(18)column(2.1 mm×150 mm,2.7μm)using 0.1%formic acid acetonitrile and 0.1%formic acid water solution as mobile phase at a flow rate of 0.3 mL/min.Mass spectrometry acquisition was performed using multiple reaction monitoring(MRM)in both positive and negative ion modes.As a result,there existed good linearities for the 10 chemical constituents in the certain concentration ranges(r>0.9970).The mean recoveries were all in the range of 94.6%~106.2%,and the relative standard deviations(RSD)were less than 5%.5 batches(24 batches in total)of Astragali Radix samples did not comply with the content regulations.Through the determination of Astragali Radix samples,the highest content of total saponins was found in ShanXi samples,the highest content of total flavonoids was found in JiLin's.By analysing the ratio of saponins/flavonoids,it has been shown that the origin influences the content of total saponins and total flavonoids in Astragali Radix.

关 键 词：高效液相色谱-串联质谱 黄芪 皂苷 黄酮 

分 类 号：TQ[化学工程]