深海真菌Phomopsis tersa FS441聚酮杂萜类化合物合成关键酶的生化功能分析  

Biochemical Functional Analysis of Key Enzyme for the Synthesis of Polyketene Heteroterpenoids from Deep-sea Fungus Phomopsis tersa FS441

作　　者：刘玉萍 李赛妮 王世新 李梦然 冯小燕 杨鑫娜 张维阳 章卫民 叶伟 李冬利[1] Liu Yuping;Li Saini;Wang Shixin;Li Mengran;Feng Xiaoyan;Yang Xinna;Zhang Weiyang;Zhang Weimin;Ye Wei;Li Dongli(Wu Yi University,Jiangmen 529020,China;Institute of Microbiology,Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangzhou 510070,China)

机构地区：[1]五邑大学,广东江门529020 [2]广东省科学院微生物研究所华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室,广东广州510070

出　　处：《广东化工》2024年第23期42-44,共3页Guangdong Chemical Industry

基　　金：广东省海洋经济发展专项资金项目([2021]054);国家自然科学基金项目(32370081);广东省科学院专项资金项目(2021GDASYL-2020103021)。

摘　　要：Phomeroids为分离自海洋真菌Phomopsis tersa FS441的聚酮杂萜类新骨架化合物,具有良好的抗肿瘤活性。前期预测ctg1509基因编码的萜类环化酶1509TC可能为负责phomeroids合成的关键酶。本研究对海洋真菌P.tersa FS441中ctg1509基因在大肠杆菌中进行表达并对1509TC蛋白用亲和层析进行纯化,并对纯化后的萜类环化酶1509TC进行体外生化功能分析。结果表明,1509TC蛋白氨基酸残基数为336,相对分子质量为38539.86 Da,是一个新型酸性亲水型萜类环化酶。1509TC能识别法尼基焦磷酸作为底物,使其转化为一个新的产物。本研究初步验证了1509TC的体外生化功能,为后期更多新型聚酮杂萜类化合物的发掘奠定了生化基础。Phomeroids are new skeleton compounds of polyketene heteroterpenoids with excellent anti-tumor activity,which are isolated from marine fungus P.tersa FS441.It is predicted that the ctg1509 gene encoding terpenoid cyclases 1509TC is responsible for the biosynthesis of phomeroids.The ctg1509 gene was expressed and in Escherichia coli and 1509TC protein was purified by affinity chromatography,the in vitro biochemical function of purified protein 1509TC were analyzed.The results indicate that the 1509 TC protein has 336 amino acid residues with a relative molecular weight of 38539.86 Da.It is a novel acidic hydrophilic terpenoid cyclase protein.1509TC can recognize farnesyl pyrophosphate as a substrate and convert it into a new product.This study preliminarily validated the in vitro biochemical function of 1509TC,laying a biochemical basis for the excavation of diverse phomeroids.

关 键 词：聚酮杂萜 异源表达 萜类环化酶 生化功能分析 

分 类 号：TQ[化学工程]