不同形状纳米银溶胶基底的增强特性比较及其对食源性芽孢的识别和定量研究  

Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores

作　　者：屠前程 田家齐 朱瑶迪 李苗云[1] 赵莉君[1] 刘世杰 梁栋 TU Qian-cheng;TIAN Jia-qi;ZHU Yao-di;LI Miao-yun;ZHAO Li-jun;LIU Shi-jie;LIANG Dong(College of Food Science and Technology,Henan Agricultural University,Zhengzhou 450000,China;Henan Jiuyuquan Food Co.,LTD.,Xinxiang 453000,China)

机构地区：[1]河南农业大学食品科学技术学院,河南郑州450000 [2]河南九豫全食品有限公司,河南新乡453000

出　　处：《分析测试学报》2024年第12期1919-1926,共8页Journal of Instrumental Analysis

基　　金：国家现代农业(肉牛牦牛)产业技术体系专项(CARS-37);国家自然科学基金(32001727);国家“十三五”重点研发计划项目(2019YF1605700);河南省重大科技专项(221100110500);河南省自然科学杰青基金(212300410008);河南省科技攻关(232102110136);河南省高校科技创新团队支持计划(22IRTSTHN021);河南省教育厅重点研发项目(22A550009);河南省科技研发计划联合基金(232103810023)。

摘　　要：该文制备并考察了3种不同形貌纳米银溶胶基底对枯草芽孢杆菌、蜡样芽孢杆菌和产气荚膜梭菌的的增强效应,并选择增强效果最优的花状纳米银(AgNF)进行表面增强拉曼散射(SERS)分析。结果显示,吡啶二羧酸钙(Ca^(2+)-DPA)在3种芽孢拉曼光谱中的出峰强度差异明显,是区别3种食源性芽孢的主要特征峰。基于SERS数据对上述3种芽孢的结构进行解析,并结合聚类分析法和K近邻算法实现了3种芽孢的快速识别。最后,通过构建枯草芽孢杆菌不同浓度对数与其1 537 cm^(-1)处SERS峰强度之间线性关系的数字化定量方程,实现了食源性芽孢的快速定量。In this paper,the enhancement effects of three kinds of nano-silver sol substrates with different morphologies on Bacillus subtilis,Bacillus cereus and Clostridium perfringens were prepared and investigated,and the flower-shaped nano-silver(AgNF) with the best enhancement effect was selected for surface enhanced Raman scattering(SERS) analysis.The results showed that the peak intensity of calcium pyridine dicarboxylate(Ca^(2+)-DPA) in Raman spectra of three kinds of spores was obviously different,which was the main characteristic peak to distinguish three kinds of food-borne spores.Based on SERS data,the structures of the above three kinds of spores were analyzed,and the rapid identification of the three kinds of spores was realized by combining cluster analysis and K nearest neighbor algorithm.Finally,by constructing the digital quantitative equation of the linear relationship between the logarithm of different concentrations of Bacillus subtilis and the SERS peak intensity at 1 537 cm^(-1),the rapid quantification of food-borne spores was realized.

关 键 词：表面增强拉曼散射 食源性芽孢 不同形状的银纳米颗粒 快速识别 数字化定量 

分 类 号：O657.3[理学—分析化学] TS201.3[理学—化学]