氨基化碳纳米管-中-四(4-羧基苯基)卟吩固载血红蛋白基电极的直接电催化性能研究  

Study on Direct Electrocatalytic Performance of Amidinated Carbon Nanotube-meso-tetrakis(4-carboxyphenyl) Porphin Supported Hemoglobin Based Electrode

作　　者：刘肖肖 曾涵[1] Liu Xiaoxiao;Zeng Han(Xinjiang Normal University,Urumqi 830054,China)

机构地区：[1]新疆师范大学,新疆乌鲁木齐830054

出　　处：《广东化工》2024年第21期10-13,共4页Guangdong Chemical Industry

基　　金：热敏碳纳米材料-二氧化硅复合物基光电传感器构效关系研究(2022D01A209)。

摘　　要：研究和发展对光敏感的电化学器件对于环境监测、环境保护和新能源的开发具有重大意义。在众多具有光响应性的纳米材料中,卟吩类有机染料因其优异的光电特性而备受青睐。使用中-四(4-羧基苯基)卟吩修饰的氨基化碳纳米管作为固定血红蛋白的载体。通过共价偶联将血红蛋白固定在纳米复合物表面,采用光谱学方法分析了酶与载体之间的相互作用。通过电化学手段,考察了固定化酶修饰电极的直接电化学行为以及对过氧化氢的催化效果。固酶电极的灵敏度为17.98μA·L1·mmol^(-1),检测限为0.003 mmol·L^(-1),米氏常数Km=0.1 mmol·L^(-1),通过光谱学和电化学方法检测了酶与载体之间的相互作用和对过氧化氢的催化作用。The research and development of light-sensitive electrochemical devices are of great significance for environmental monitoring,environmental protection and new energy development.Among many photoresponsive nano-materials,porphin organic dyes are favored because of their excellent photoelectric properties.Amino modified carbon nanotubes modified by meso-tetrakis(4-carboxyphenyl) porphyrin were used as the carrier to fix hemoglobin.Hemoglobin was fixed on the surface of nanocomplex by covalent coupling,and the interaction between enzyme and carrier was analyzed by spectroscopy.The direct electrochemical behavior of immobilized enzyme modified electrode and its catalytic effect on hydrogen peroxide was investigated by electrochemical means.The sensitivity of the solid enzyme electrode is 17.98 μA·mmol^(-1)·L^(-1),the detection limit was 0.003 mmol·L^(-1),and the Mi constant km=0.1 mmol·L^(-1).The interaction between the enzyme and the carrier and the catalytic effect on hydrogen peroxide were detected by spectroscopy and electrochemical methods.

关 键 词：血红蛋白 纳米复合材料 中-四-(4-羧基苯基)卟吩 过氧化氢 相互作用 

分 类 号：TQ[化学工程]