高效液相色谱测定饮用水中16种多环芳烃的方法优化  

Optimization of the Method for Determining 16 Polycyclic Aromatic Hydrocarbons in Drinking Water by High-Performance Liquid Chromatography

作　　者：钟建军 张琪雨 吴艳龙 秦伟 王宏宇 Zhong Jianjun;Zhang Qiyu;Wu Yanlong;Qin Wei;Wang Hongyu(Zhuhai Water Supply and Drainage Pollution Control Center,Zhuhai 519000,China)

机构地区：[1]珠海市供水与排水治污中心,广东珠海519000

出　　处：《广东化工》2024年第21期152-155,148,共5页Guangdong Chemical Industry

摘　　要：使用经过优化后的固相萃取-高效液相色谱,结合二极管阵列检测器串联荧光检测器的高效液相色谱法,测定生活饮用水中的16种多环芳烃(Polycyclic Aromatic Hydrocarbons,PAHs)。通过优化流动相流速、梯度洗脱程序方案以及检验波长,实现了经二极管阵列检测器/荧光检测器串联后对16种特定多环芳烃化合物的分离和检测。实验结果表明,标准曲线线性关系良好,相关系数均高于0.995;方法检出限范围为0.5 ng/L~4.8 ng/L,测定下限范围为2.0 ng/L~19.2 ng/L;相对标准偏差RSD整体范围介于0.13%~7.4%之间;验证低、中、高浓度相对误差范围分别介于-1.3%~7.3%,-4.9%~2.6%,-4.8%~4.2%之间;整体加标回收率范围介于77.2%~109%之间,符合生活饮用水中16种多环芳烃的分析检测要求。Utilizing an optimized solid phase extraction-high performance liquid chromatography(SPE-HPLC) method equipped with a diode array detector coupled in series with a fluorescence detector,designed for the analysis of 16 Polycyclic Aromatic Hydrocarbons(PAHs) in drinking water.By refining the mobile phase flow rate,gradient elution program,and the detection wavelengths,this approach achieves successful separation and detection of the 16 specific PAH compounds.Experimental outcomes demonstrate a favorable linear relationship of the standard curves,with correlation coefficients exceeding 0.995.The detection limits range from 0.5 ~ 4.8 ng/L,while the quantitation limits span from 2.0 ng/L^(-1)9.2 ng/L.The relative standard deviations(RSD) across all measurements are within the scope of 0.13 %~7.4 %.Accuracy assessments revealed relative errors at low,medium,and high concentration levels varying between-1.3 % ~7.3 %,-4.9 % ~2.6 %,and-4.8 %~4.2 %,respectively.Furthermore,the recovery rates of spiked samples fall between 77.2 % and 109 %,comprehensively meeting the analytical requirements for the determination of 16 PAHs in drinking water.This methodology thus offers a reliable and sensitive tool for the monitoring of PAH contaminants in drinking water supplies.

关 键 词：多环芳烃 高效液相色谱 生活饮用水 固相萃取 方法优化 

分 类 号：TQ[化学工程]