基于UHPLC-Q-Exactive Orbitrap MS技术快速鉴定血水草的化学成分  

Rapid Identification of Chemical Components of Eomecon Chionantha Hance based on UHPLC-Q-Exactive Orbitrap MS

作　　者：马洁瑶 彭浩 吴雅澜 蒲沁琳 马廷升 Ma Jieyao;Peng Hao;Wu Yalan;Pu Qinlin;Ma Tingsheng(Hunan University of Medicine,Huaihua 418000,China)

机构地区：[1]湖南医药学院,湖南怀化418000

出　　处：《广东化工》2024年第21期159-163,169,共6页Guangdong Chemical Industry

基　　金：湖南省自然科学基金项目(2022JJ50293)。

摘　　要：血水草(Eomecon chionantha Hance)为罂粟科血水草属的多年生草本植物,其全草入药,具有清热解毒、消炎止痛等功效,但其功效性物质基础尚未被完全揭示。本研究建立一种高效快速的超高效液相色谱串联四极杆轨道阱高分辨质谱法(UHPLC-Q-Exactive Orbitrap MS),对血水草的化学成分进行表征。使用Thermo scientific Hypersil GLOD aQ色谱柱(1.9μm,2.1 mm ID×100 mm),以流动相0.1%甲酸水溶液(A)-乙腈(B)进行梯度洗脱;流速为0.3 mL·min^(-1),检测波长284 nm,进样量为1μL,柱温为35℃。质谱采用电喷雾离子源(ESI)正离子模式,质谱扫描范围为m/z 100~1500。通过数据库及参考文献共鉴定出血水草中73个化学成分,其中包括异喹啉类生物碱33个,酰胺类化合物10个,氨基酸及其衍生物5个,萜类4个,黄酮类2个,其他19个。所建立的方法能够高效、快速地鉴定血水草的化学成分,为血水草药效物质基础研究和资源开发利用提供依据。Eomecon chionantha Hance,a perennial herb belonging to the genus Eomecon within the family Papaveraceae,is used medicinally as a whole plant.Owing to the lack of comprehensive techniques to characterize its chemical components.Thus,its development and further promotion are limited.In this study,a reliable strategy based on Ultra-high performance liquid chromatography tandem hybrid quadrupole-orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap MS)was established to rapidly screen and analyze the main chemical components of Eomecon chionantha.The type of column was Hypersil GOLD aQ column(1.9 μm,2.1 mm ID × 100 mm).The mobile phases were 0.1 % formic acid-water(liquid A) and 0.1 % acetonitrile(liquid B).The flow rate was 0.3 mL·min^(-1),the column temperature was 35 ℃,and the injection volume was 1 μL.The results show that 73 compounds are identified from Eomecon chionantha,including 33 isoquinoline alkaloids,10 amide compounds,5 amino acids and their derivatives,4 terpenes,2 flavonoids,and 19 others.The established method can efficiently and rapidly identify the chemical constituents of Eomecon chionantha,providing a basis for research on the active substances and the development and resources utilization of Eomecon chionantha.

关 键 词：血水草 高分辨质谱 化学成分 

分 类 号：TQ[化学工程]