不同配比黄芪-川芎方中核苷测定及其灰色关联度分析  

Determination and grey relational analysis of nucleosides in Astragalus-Chuanxiong decoction with differ-ent proportions

作　　者：侯月彩 薛莹[1,2] 青琳森[1] 梁健[1] 雍莉[2] HOU Yuecai;XUE Ying;QING Linsen;LIANG Jian;YONG Li(Chengdu Institute of Biology,Chinese Academy of Sciences,Sichuan Chengdu 610213,China;Sichuan Center for Disease Control&.Prevention,Sichuan Chengdu 610041,China)

机构地区：[1]中国科学院成都生物研究所,四川成都610213 [2]四川省疾病预防控制中心,四川成都610041

出　　处：《中国医院药学杂志》2024年第22期2598-2605,共8页Chinese Journal of Hospital Pharmacy

基　　金：中国科学院战略生物资源服务网络计划(编号:KFJ-BRP-008-007);四川省科技计划(编号:2022YFH0117)。

摘　　要：目的:建立UPLC-MS/MS方法同时测定黄芪-川芎共煎液12个核苷类化合物的含量,并结合灰色关联度法优选药对最佳配伍比例。方法:采用Thermo Hypercard色谱柱(2.1 mm×100 mm,5μm),流动相为水(含2 mmol·L^(-1)乙酸铵、0.06%乙二胺)(A)-乙腈(含2 mmol·L^(-1)乙酸铵)(B),梯度洗脱,流速为0.6 mL·min^(-1),柱温为40℃,进样量为10μL。负离子模式测定药对不同比例共煎液鸟苷、黄苷、尿苷、胞苷、腺苷、胸苷、肌苷、鸟嘌呤核苷酸、腺嘌呤核苷酸、尿嘧啶核苷酸、胞嘧啶核苷酸、脱氧鸟苷12个成分含量,并通过灰色关联度综合分析含量测定结果。结果:12个核苷类成分在18 min内达到分离,线性关系良好(R^(2)≥0.9994),精密度、稳定性、重复性试验符合要求,平均回收率在97.19%~101.2%,RSD≤4.93%。黄芪-川芎配比为1:3共煎时核苷类成分的提取率最高。结论:所建方法简便可行、灵敏可靠,可用于黄芪-川芎的核苷类成分含量测定,及优选最佳药对配伍比例。OBJECTIVE To establish a UPLC-MS/MS method for simultaneous determination of 12 nucleoside compounds in Astragalus-Chuanxiong decoction(ACD)and optimize the optimal compatibility ratio of drug pairs through combining grey cor-relation method.METHODS Thermo Hypercard column(2.1 mm×100 mm,5μm)was utilized for analysis.The mobile phase was composed of water(containing 2 mmol·L^(-1) ammonium acetate and 0.06%ethylenediamine)(A)-acetonitrile(contain-ing 2 mmol·L^(-1) ammonium acetate)(B)(gradient elution).Flow rate was 0.6 mL·min^(-1),column temperature 40℃and sample size 10μL.Negative ion mode was employed for determining the contents of 12 components including guanosine,xanthine,uri-dine,cytidine,adenosine,thymidine,inosine,guanine nucleotide,adenine nucleotide,uracil nucleotide,cytosine nucleotide and deoxyguanosine in different proportions of water decoction.The results were analyzed in combination with grey correlation degree content determination.RESULTS Twelve nucleosides were separated within 18 min with an excellent linearity(R^(2)≥0.9994).Precision,stability and repeatability tests were decent with an average recovery rate of 97.19%-101.2%and RSD≤4.93%.Extraction rate of nucleosides peaked with a ratio of ACD 1∶3.CONCLUSION The established method is simple,feasible,sen-sitive and reliable.It may be used for quantifying nucleoside compounds in ACD.And drug pairing ratio is optimized.

关 键 词：核苷 黄芪-川芎 配伍 共煎 灰色关联度 

分 类 号：R917[医药卫生—药物分析学]