润目汤熏蒸对水液缺乏型干眼兔泪液分泌、角膜泪腺形态及炎症相关蛋白表达水平的影响  

Effects of Runmu Decoction steaming on tear secretion,corneal and lacrimal gland morphology and expressions of inflammation-related proteins in rabbits with aqueous-deficient dry eye

作　　者：谢思健[1] 马小丫 唐逸飞 杜佳琪 冯娴 赖娟[1] 伍竟涛 姜尚萍 彭清华[1] XIE Sijian;MA Xiaoya;TANG Yifei;DU Jiaqi;FENG Xian;LAI Juan;WU Jingtao;JIANG Shangping;PENG Qinghua(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Xi'an Jiaotong University,Xi'an,Shaanxi 710049,China;The Third Xiangya Hospital of Central South University,Changsha,Hunan 410013,China;School of Medicine,Xiamen University,Xiamen,Fujian 361023,China;Ningxiang Hospital of Chinese Medicine,Changsha,Hunan 410000,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]西安交通大学,陕西西安710049 [3]中南大学湘雅三医院,湖南长沙410013 [4]厦门大学医学院,福建厦门361023 [5]宁乡市中医医院,湖南长沙410000

出　　处：《湖南中医药大学学报》2024年第11期1991-1998,共8页Journal of Hunan University of Chinese Medicine

基　　金：湖南省教育厅科学研究项目(21C0249);湖南中医药大学校级科研基金项目(Z2023XJYB24);湖南中医药大学本科生科研创新基金项目(2024BKS005)。

摘　　要：目的观察润目汤中药熏蒸对水液缺乏型干眼兔泪液分泌、角膜泪腺形态及炎症相关蛋白表达水平的影响。方法取健康新西兰大白兔30只,随机分为5组(空白对照组、蒸馏水组、阳性对照组、联合治疗组和中药熏蒸组),每组6只。空白对照组不予处理,其余各组用1%硫酸阿托品眼用凝胶连续滴眼3 d。造模成功后(实验第4天开始),空白对照组在正常环境中常规喂养。蒸馏水组给予中药熏蒸治疗仪加热蒸馏水熏蒸眼部对照处理;阳性对照组予以玻璃酸钠滴眼液滴眼,1次1滴,4次/d;联合治疗组采用润目汤熏蒸和玻璃酸钠滴眼液联合滴眼治疗;中药熏蒸组采用中药熏蒸治疗仪加热润目汤水煎液熏蒸眼部,1次/d,15 min/次。同时各组均滴1%硫酸阿托品滴眼液以维持模型,直至实验结束。在实验第0、3、7、10、13天分别进行泪液分泌试验(SchirmerⅠtest,SⅠT)、角膜荧光素钠染色(corneal fluorescein staining,CFS)评分;第3、13天进行角膜共聚焦显微镜检查;第13天处死动物。在光镜下观察角膜上皮形态学变化,电镜下观察角膜上皮及泪腺组织形态学变化;采用Western blot检测角膜组织中白细胞介素-1α(interleukin-1α,IL-1α)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达。结果与空白对照组比较,在第7、10天,蒸馏水组和联合治疗组SⅠT降低(P<0.01),各组CFS评分升高(P<0.05);在第13天,蒸馏水组SⅠT降低(P<0.01),蒸馏水组和阳性对照组CFS评分升高(P<0.05)。与蒸馏水组比较,在第7天,阳性对照组和中药熏蒸组SⅠT升高(P<0.01),联合治疗组CFS评分降低(P<0.05);在第10、13天,阳性对照组、中药熏蒸组SⅠT升高(P<0.01)、CFS评分降低(P<0.01)。与阳性对照组比较,在第7天,联合治疗组SⅠT降低(P<0.05);在第10天,联合治疗组CFS升高(P<0.05)。与联合治疗组比较,在第7、10天,中药熏蒸组SⅠT升高(P<0.05)。与空白对照组比较,阳性对照组、中药Objective To observe the effects of Runmu Decoction steaming on tear secretion,corneal and lacrimal gland morphology,and expression levels of inflammation-related proteins in rabbits with aqueous-deficient dry eye.Methods Thirty healthy New Zealand white rabbits were randomized into blank control group,distilled water group,positive control group,combined treatment group,and Chinese medicine steaming group,with six rabbits in each group.The blank control group received no treatment,while the remaining groups were treated with 1%atropine sulfate ophthalmic gel in the eyes for 3 consecutive days.After successful modeling(starting from the 4th day of the experiment),the blank control group was fed routinely in a normal environment.The distilled water group was given distilled water that had been heated by a Chinese medicine steaming apparatus for steaming the eyes as a control treatment.The positive control group was treated with sodium hyaluronate eye drops,one drop each time,four times a day.The combined treatment group was treated with both steaming with Runmu Decoction and sodium hyaluronate eye drops.The Chinese medicine steaming group was treated with Runmu Decoction that had been heated for steaming the eyes using the Chinese medicine steaming apparatus for 15 minutes each time,once a day.Meanwhile,1%atropine sulfate eye drops were administered to all groups to maintain the model until the end of the experiment.Schirmer I test(SIT)and corneal fluorescein sodium staining(CFS)scoring were conducted on the 0th,3rd,7th,10th,and 13th days of the experiment.Corneal confocal microscopy was performed on the 3rd and 13th days.The animals were sacrificed on the 13th day.The morphological changes of the corneal epithelium were observed using a light microscope,and the histomorphological changes of the corneal epithelium and lacrimal glands were observed using an electron microscope.The expression levels of interleukin-1α(IL-1α)and tumor necrosis factor-α(TNF-α)in the corneal tissue were measured by Western blot.Resu

关 键 词：润目汤 中药熏蒸 水液缺乏型干眼 炎症因子 白细胞介素-1Α 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]