A Biomimetic Membrane-Coated Nanoprobe for the Ratiometric Fluorescence Detection of Phospholipase A_(2)  

作　　者：Xingyu He Yuanyuan Dong Pan He Chenghui Liu Wei Ren 

机构地区：[1]Key Laboratory of Applied Surface and Colloid Chemistry,Ministry of Education,Xi’an,710119,China [2]Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province,Xi’an,710119,China [3]School of Chemistry&Chemical Engineering,Shaanxi Normal University,Xi’an,710119,China

出　　处：《Journal of Analysis and Testing》2024年第3期270-277,共8页分析检测（英文）

基　　金：supported by the National Natural Science Foundation of China(21904083);the Program for Changjiang Scholars and Innovative Research Team in University(IRT_15R43);the Fundamental Research Funds for the Central Universities(GK202201009,GK202101001,and GK202305001);the Innovation Capability Support Program of Shaanxi Province(2021TD-42).

摘　　要：Phospholipase A_(2)(PLA_(2))is a critical biomarker of many diseases.Among various fluorescent probes,the ratiometric ones have shown great promise because they can provide built-in calibration to avoid interference from analyte-independent factors by simultaneously monitoring two or more fluorescence peaks.Herein,we develop a biomimetic membrane-coated ratiometric fluorescent nanoprobe for the detection of PLA_(2).In this design,silica nanoparticles modified with fluorescein isothiocyanate(FITC)are functionalized with a layer of biomimetic membrane that is loaded with Dil dye.Therefore,when the nanocomposite is illuminated by 488 nm incident light,in addition to the fluorescence of FITC centered at 522 nm,that of Dil peaked at 568 nm originating from the energy transfer from FITC to Dil can also be monitored,forming a ratiometric fluorescent nanoprobe.When the target PLA_(2)is added,FITC and Dil will no longer be in close proximity because it hydrolyzes the biomimetic membrane,cutting off the energy flow between the two dyes.Consequently,the ratiometric fluorescent signal of the nanoprobe is altered to quantitatively reflect the content of PLA_(2).This study provides an effective approach to PLA_(2)detection,and the biomimetic membrane-mediated ratiometric design is promising in developing a new generation of biosensors.

关 键 词：Biomimetic membrane Ratiometric nanoprobe Energy transfer Phospholipase A_(2) 

分 类 号：O657.3[理学—分析化学] R446[理学—化学]