淋巴细胞亚群对儿童传染性单核细胞增多症的临床意义  

Clinical significance of lymphocyte subsets in children with infectious mononucleosis

作　　者：樊书 冯同保 FAN Shu;FENG Tongbao(Department of Clinical Laboratory,the Second People′s Hospital of Changzhou Affiliated to Nanjing Medical University,Changzhou,Jiangsu 213000,China)

机构地区：[1]南京医科大学附属常州第二人民医院医学检验科,江苏常州213000

出　　处：《检验医学与临床》2024年第S02期15-20,共6页Laboratory Medicine and Clinic

基　　金：江苏省常州市科技支撑社会发展项目(CE20235061)。

摘　　要：目的研究淋巴细胞亚群对儿童传染性单核细胞增多症(IM)的临床应用价值。方法选取2022年2月至2023年11月该院确诊的儿童传染性单核细胞增多症患者96例作为IM组,另外收集在此期间体检的75例健康儿童作为对照组,对两组病例进行血常规、异型淋巴细胞计数及外周血淋巴细胞亚群检测,对两组检验结果进行分析。结果与对照组相比,IM组患者的白细胞总数、淋巴细胞绝对值、单核细胞绝对值、淋巴细胞百分率、异型淋巴细胞百分率、CD3^(+)T淋巴细胞百分比和CD8^(+)T淋巴细胞百分比增高,差异有统计学意义(P<0.05);中性粒细胞绝对值、中性粒细胞百分率、单核细胞百分率、CD4^(+)T淋巴细胞百分比、CD19^(+)B淋巴细胞百分比、CD16^(+)CD56^(+)自然杀伤细胞百分比和CD4^(+)/CD8^(+)比值降低,差异具有统计学意义(P<0.05)。异型淋巴细胞百分比与CD4^(+)T淋巴细胞百分比和CD4^(+)/CD8^(+)比值呈负相关(r=-0.46、-0.49,P<0.05);与CD8^(+)T淋巴细胞百分比呈正相关(r=0.66,P<0.05);CD4^(+)CD8^(+)T淋巴细胞(DPT细胞)百分比显著高于对照组(P<0.05)。结论IM患者淋巴细胞亚群水平均出现明显异常,并且异型淋巴细胞百分率与CD3^(+)T淋巴细胞、CD8^(+)T淋巴细胞百分比及CD4^(+)/CD8^(+)比值均有明显相关性,因此淋巴细胞亚群检测为辅助临床诊断IM提供了理论依据。Objective To investigate the clinical application value of lymphocyte subsets in children with infectious mononucleosis(IM).Methods A total of 96 children with infectious mononucleosis diagnosed in the hospital from February 2022 to November 2023 were selected as the IM group,and 75 healthy children who underwent physical examination during this period were collected as the control group.Blood routine,blood cell classification and peripheral blood lymphocyte subsets were detected for the two groups of cases.The test results of the two groups were analyzed.Results Compared with the control group,the total number of white blood cells,absolute value of lymphocytes,absolute value of monocytes,percentage of lymphocytes,percentage of atypical lymphocytes,percentage of CD3^(+)T lymphocytes and percentage of CD8^(+)T lymphocytes in the IM group were increased,with statistical significance(P<0.05).The absolute value of neutrophils,percentage of neutrophils,percentage of monocytes,percentage of CD4^(+)T lymphocytes,percentage of CD19^(+)B lymphocytes,percentage of CD16^(+)CD56^(+)natural killer cells and CD4^(+)/CD8^(+)ratio were decreased,and the differences were statistically significant(P<0.05).The percentage of atypical lymphocytes was negatively correlated with the percentage of CD4^(+)T lymphocytes and the ratio of CD4^(+)/CD8^(+)(r=-0.46,-0.49,P<0.05).It was positively correlated with the percentage of CD8^(+)T lymphocytes(r=0.66,P<0.05).The percentage of double positive T cell(DPT)of CD4^(+)CD8^(+)was significantly higher than that of control group(P<0.05).Conclusion The level of lymphocyte subsets in IM patients was obviously abnormal,and the percentage of allotypic lymphocytes was significantly correlated with the percentage of CD3^(+)T lymphocytes,CD8^(+)T lymphocytes and the ratio of CD4^(+)/CD8^(+).Therefore,the detection of lymphocyte subsets provided a theoretical basis for assisting clinical diagnosis of IM.

关 键 词：淋巴细胞亚群 儿童 传染性单核细胞增多症 EB病毒 流式细胞术 

分 类 号：R725.1[医药卫生—儿科]