南京梅山地区消化病患者幽门螺杆菌抗体谱分析  

Analysis of antibody detection results of Helicobacter pylori in gastroenterology department

作　　者：余月嫚 徐静 徐天时 孙士佳 产松苗 杨侠宇 YU Yueman;Xu Jing;XU Tianshi;SUN Shijia;CHAN Songmiao;YANG Xiayu(Department of Clinical Laboratory,Nanjing Meishan Hospital,Nanjing,Jiangsu 210039,China;Department of Gastroenterology,Nanjing Meishan Hospital,Nanjing,Jiangsu 210039,China)

机构地区：[1]南京梅山医院检验科,江苏南京210039 [2]南京梅山医院消化科,江苏南京210039

出　　处：《检验医学与临床》2024年第S02期69-73,共5页Laboratory Medicine and Clinic

摘　　要：目的研究南京梅山地区消化病患者幽门螺旋杆菌(Hp)抗体的阳性率及其在不同年龄组与性别之间的差异,引导临床了解本地区人群患病概况,从而为不同年龄、不同性别的人群提供预防与诊断依据。方法选取2019年10月至2022年6月期间该院2564例消化科患者血清,将年龄分为<18岁、18~<45岁、45~<60岁和≥60岁四组,根据性别分为男、女两组。采用免疫印迹法检测Hp抗体:抗CagA抗体、抗VacA抗体、抗UreA抗体和抗UreB抗体,并对数据进行χ^(2)检验。结果在2564例消化科就诊患者中,1529例为Hp阳性,感染率为59.6%,Hp感染主要以Hp-Ⅱ型为主,Hp-Ⅰ型和Hp-Ⅱ型感染比较差异有统计学意义(P<0.01);18~<45岁组、45~<60岁组和≥60岁组均以VacA抗体阳性率最低,比较差异均有统计学意义(P<0.01);VacA阳性、UreA阳性和UreB阳性组的年龄段差异均有统计学意义(P<0.01);感染Hp的男726例(53.9%)、女803例(65.9%),男性和女性均是Hp-Ⅱ型感染率高于Ⅰ型;UreB抗体阳性率最高、VacA抗体阳率最低,差异均有统计学意义(P<0.01)。结论消化道疾病的发生与感染Hp有关,对Hp进行分型检测对消化道疾病程度的判断、预后及诊疗有重要的指导意义,并可防止耐药性的产生。Objective To study the positive rate of Helicobacter pylori(Hp)antibody in patients with digestive diseases in Meishan area of Nanjing and its differences between different age groups and sexes,guide the clinical understanding of the epidemic profile of the population in the region,so as to provide prevention and diagnosis basis for people of different ages and genders.Methods From October 2019 to June 2022,2564 serum samples were collected from the department of gastroenterology in the hospital.The patients were divided into four groups:<18 years old,18 to<45 years old,45 to<60 years old and≥60 years old.The Helicobacter pylori antibodies:anti-CagA,anti-VacA,anti-UreA and anti-UreB,were detected by western blotting and the data were analyzed byχ^(2) test.Results Among 2564 patients in gastroenterology department,1529 patients were Hp positive,and the infection rate was 59.6%,Hp infection was mainly characterized by Hp-type-Ⅱ,There was significant difference between Hp-type-Ⅰand Hp-type-Ⅱinfection(P<0.01);the positive rate of VacA antibody was the lowest in the group 18 to<45 years old,the group 45 to<60 years old,and the group≥60 years old,and the differences were statistically significant(P<0.01).There was significant difference in VacA positive group,UreA positive group and UreB positive group(P<0.01).There were 726 males(53.9%)and 803 females(65.9%)infected with Hp,and the infection rate of Hp-type-Ⅱwas higher than that of Hp-type-Ⅰin both males and females,the positive rate of UreB antibody was the highest and that of Vaca antibody was the lowest,and the differences were statistically significant(P<0.01).Conclusion The occurrence of digestive tract diseases is related to infection with Hp,and the classification and detection of Hp has important guiding significance for the judgment,prognosis,diagnosis and treatment of digestive tract diseases,and can prevent the emergence of drug resistance.

关 键 词：幽门螺杆菌 Hp-Ⅰ型 Hp-Ⅱ型 抗CagA抗体 抗VacA抗体 抗UreA抗体 抗UreB抗体 

分 类 号：R446.6[医药卫生—诊断学]