1例微柱凝胶法血型鉴定产生拖尾现象研究  

A study on a case of trailing phenomenon in blood type Identification using microgel column method

作　　者：赵辉 厉淑红 吕玉 李瑶 张雯雯 ZHAO Hui;LI Shuhong;LV Yu;LI Yao;ZHANG Wenwen(Department of Out-patient,Jinan International Travel Healthcare Center,Jinan,Shandong 250014,China)

机构地区：[1]山东省济南国际旅行卫生保健中心(济南海关口岸门诊部),山东济南250014

出　　处：《检验医学与临床》2024年第S02期98-99,共2页Laboratory Medicine and Clinic

摘　　要：目的结合血清学检测及基因分型,探讨影响微柱凝胶法血型检测出现拖尾现象结果判定的原因及血型基因筛查的意义,为提高血型检测的准确性提供个案依据。方法从血液标本采集、红细胞悬液制备、检测方法学原理等方面调查产生拖尾现象的原因。结果产生拖尾现象的原因与血标本离心时间、生理盐水质量、红细胞悬液配制浓度不当等因素有关。结论血型检测从标本采集到结果判读是一个完整的过程,应严格控制每个环节的工作质量,避免人为等各种因素对检测过程和结果的影响。Objective Combined with serological detection and genotyping,this paper discusses the reasons affecting the determination of tailing phenomenon in blood group detection by microgel column method and the significance of blood group gene screening,so as to provide a case basis for improving the accuracy of blood type testing.Methods The causes of tailing phenomenon were investigated from the aspects of the collection of blood samples,preparation of red cell suspension and principle of detection methodology.Results The reasons of tailing phenomenon are related to centrifugation time of blood samples,quality of normal saline and improper concentration of red cell suspension.Conclusion Blood type testing is a complete process from specimen collection to result interpretation.The quality of each link should be strictly controlled to avoid the influence of human factors on the detection process and results.

关 键 词：血型检测 拖尾现象 原因分析 微柱凝胶法 

分 类 号：R446.6[医药卫生—诊断学]