福建省典型杉木人工林根际土壤氧化亚氮还原菌丰度特征  

Gene Abundance of N_(2)O Reducers in Rhizosphere Soils of Typical Chinese Fir Plantations from Fujian Province

作　　者：左婷 孙其荣 陈婧涵 罗培傑 吴黄垒 郑勇 林永新 叶桂萍 ZUO Ting;SUN Qirong;CHEN Jinghan;LUO Peijie;WU Huanglei;ZHENG Yong;LIN Yongxin;YE Guiping(College of Geography and Oceanography,Minjiang University,Fuzhou 350108,China;School of Geographical Sciences,Fujian Normal University,Fuzhou 350007,China;Technology Innovation Center for Monitoring and Restoration Engineering of Ecological Fragile Zone in Southeast China,Ministry of Natural Resources,Fuzhou 350001,China)

机构地区：[1]闽江学院地理与海洋学院,福州350108 [2]福建师范大学地理科学学院,福州350007 [3]自然资源部东南生态脆弱区监测修复工程技术创新中心,福州350001

出　　处：《土壤》2024年第5期992-998,共7页Soils

基　　金：国家自然科学基金项目(31971447);福建省自然资源科技创新项目(KY-090000-04-2022-012);闽江学院引进人才预研项目(MJY20012)资助。

摘　　要：为揭示福建省典型杉木人工林根际土壤中氧化亚氮(N_(2)O)还原菌的丰度及其关键环境驱动因子,选取3个典型林场的杉木人工林为对象,利用实时荧光定量PCR测定了根际土壤N_(2)O还原菌功能基因nosZⅠ和nosZⅡ丰度。结果表明:3个典型杉木人工林根际土壤的nosZⅠ基因丰度均显著高于nosZⅡ,表明nosZⅠ在酸性杉木人工林根际土壤中占主导,这可能与nosZⅡ型N_(2)O还原菌对低pH比较敏感有关;3个林场中,武夷山国家森林公园的杉木人工林根际土壤nosZⅠ基因丰度为2.53×10^(7)copies/g,显著高于三明国家野外科学观测研究站(9.27×10^(6) copies/g)和上杭白砂国有林场(8.90×10^(6) copies/g),但3个林场之间的nosZⅡ丰度差异不显著;随机森林分析表明,土壤pH是调控N_(2)O还原菌nosZⅠ和nosZⅡ丰度的关键环境因子。综上所述,福建省不同区域典型杉木人工林根际土壤nos ZⅠ基因丰度存在差异,土壤pH是最主要的调控因子。In order to reveal the abundance of N_(2)O reducers and their critical driving factors in the rhizosphere soils of typical Chinese fir plantation from Fujian Province,rhizosphere soil samples under Chinese fir plantation from three typical forest farms in Fujian Province were collected.The abundance of functional genes of soil N_(2)O reducers,namely nosZⅠand nosZⅡ,were determined using real-time quantitative PCR.Results showed that the abundance of nosZⅠgene was significantly higher than that of nosZⅡin the rhizosphere soils of Chinese fir plantation in all the three typical forest farms,which suggested that nosZⅠ-type N_(2)O reducers dominate in the acidic rhizosphere soils of Chinese fir plantation,possibly due to the fact that the nosZⅡ-type N_(2)O reducers are more sensitive to low pH conditions.Among the three forest farms,the abundance of nosZⅠgene was 2.53×107 copies/g in Wuyishan National Park,and significantly higher than 9.27×10^(6)copies/g in Sanming Forest Ecosystem National Observation and Research Station and 8.90×10^(6) copies/g in Shanghang Baisha Stated-owned Forest Farm.However,there was not significant difference in nosZⅡabundance among the three forest farms.Random forest analysis showed that soil pH was the key environmental factor regulating the abundance of nosZⅠand nosZⅡ.In conclusion,the abundance of nosZⅠgene varies substantially in the rhizosphere soils of Chinese fir plantation from three typical forest farms in different regions of Fujian Province,while soil pH is the most important driving factor.

关 键 词：森林土壤 根际土壤 nosZⅠ nosZⅡ 

分 类 号：S154.36[农业科学—土壤学]