Sensitivity of miRNA-181a,miRNA-23b and miRNA-16 in the Late-Onset Neonatal Sepsis:A Diagnostic Study  

作　　者：Divya Katta Kopula Satyamoorthy Sridharan Uma Maheswari Balakrishnan Prakash Amboiram Kennedy Kumar 

机构地区：[1]Department of Microbiology,Sri Ramachandra Institute of Higher Education and Research,Porur,Chennai 600116,India [2]Department of Neonatology,Sri Ramachandra Institute of Higher Education and Research,Porur,Chennai 600116,India

出　　处：《Maternal-Fetal Medicine》2024年第4期243-248,共6页母胎医学杂志（英文）

基　　金：University Grants Commission Rajiv Gandhi National Fellowship.

摘　　要：Objective: To evaluate the expression and diagnostic value of levels of the microRNAs(miRNAs),miRNA-181a,miRNA-23b,and miRNA-16,in late-onset neonatal sepsis(LOS)and compare them with the diagnostic utility of C-reactive protein(CRP)levels.Methods: This was a prospective diagnostic study conducted between January 2021 and March 2023 at a tertiary care center(Sri Ramachandra Hospital)in India.Quantitative real-time polymerase chain reaction was performed to determine miRNA-181a,miRNA-23b,and miRNA-16 expression levels,and CRP was measured by nephelometry.The diagnostic value of miRNA and CRP levels were analyzed using receiver operating characteristic(ROC)curves.ROC curves were utilized to determine optimal cutoff points,and Mann–Whitney tests were performed using SPSS to ascertain P values,with statistical significance defined as<0.05.Results: This study included 100 samples,with 50 cases of culture-proven LOS(27 females,23 males)and 50 healthy controls(31 females,19 males).In LOS,miRNA-181a and miRNA-23b expression levels were significantly downregulated(P<0.001),with area under the curve(AUC)values of 0.83 and 0.92,respectively,whereas those of miRNA-16 were significantly upregulated(P<0.001;AUC=0.97).In comparison,CRP levels had an AUC value of 0.831(P<0.001).Further,miRNA-23b showed the highest sensitivity(98%)of markers tested,whereas miRNA-16 exhibited the highest specificity(96%).Conclusion: MiRNA,especially miRNA-16,shows diagnostic potential for neonatal sepsis compared with traditional biomarkers like CRP and procalcitonin,suggesting its use as an early marker for LOS.However,further cohort studies are needed before practical application.

关 键 词：MICRORNAS Real-time PCR Blood culture Biomarkers Late-onset sepsis 

分 类 号：R722.1[医药卫生—儿科]