枯草芽孢杆菌黑色变种选择性培养基优化  

Optimization of Bacillus subtilis var.niger selective medium

作　　者：郁斯贻 龚海燕 YU Siyi;GONG Haiyan(Shanghai Environmental Monitoring Center,Shanghai 200235,China)

机构地区：[1]上海市环境监测中心,上海200235

出　　处：《安徽农学通报》2024年第23期40-45,共6页Anhui Agricultural Science Bulletin

基　　金：上海市科技委员会项目“养殖业消毒副产物环境监测与重大疫情消毒应急预案研究”(21DZ1202202);上海市生态环境局青年科研项目(沪环青科〔2023〕第7号)。

摘　　要：为筛选能使枯草芽孢杆菌黑色变种稳定产生黑色素的酪氨酸培养基配方,本试验通过单因素实验法对酪氨酸培养基进行优化,探讨酪氨酸(L-酪氨酸,浓度分别为0、0.5、1.0、2.0和3.0g/L)、碳源(葡萄糖,浓度分别为0、0.5、1.0、2.0和3.0 g/L)、氮源(胰蛋白胨,浓度分别为0.5、1.0、2.0、3.0、4.0和5.0 g/L)、氨基酸(干酪素,浓度分别为0、10.0、20.0、40.0和60.0 mL/L)及诱导时间(24、48、72和96 h)对枯草芽孢杆菌黑色变种产生黑色素的影响,并对富捷牌环氧乙烷灭菌ATCC 9372生物指示剂等4种指示剂进行适应性验证。结果表明,L-酪氨酸、葡萄糖、胰蛋白胨和干酪素的最优添加量分别为0.5、0.5、0.5g/L和10mL/L;最优培养时间在72~96 h。该条件下能较好地诱导枯草芽孢杆菌黑色变种稳定产生黑色素。研究结果为枯草芽孢杆菌黑色变种的筛选、培养及进一步应用提供参考。To screen the tyrosine medium formula that could make Bacillus subtilis var.niger produce melanin stably,the tyrosine medium was optimized by single factor experiment method,and the effects of Tyrosine(L-tyrosine,0,0.5,1.0,2.0 and 3.0 g/L,respectively),carbon source(glucose,0,0.5,1.0,2.0 and 3.0 g/L,respectively),nitrogen source(tryptone,1.0,2.0 and 3.0 g/L,respectively),amino acid(casein,0,10.0,20.0,40.0 and 60.0 mL/L,respectively)and induction time(24,48,72 and 96 h)on the production of melanin by Bacillus subtilis var.niger were investigated.The adaptability of 4 kinds of indicators,including ATCC 9372 biological indicator sterilized by Fujie brand ethylene oxide,was verified.The results showed that the optimal addition amounts of L-tyrosine,glucose,tryptone and casein were 0.5,0.5,0.5 g/L and 10 mL/L,respectively.The optimal culture time was 72~96 h.Under this condition,the Bacillus subtilis var.niger could be induced well,and the melanin produced was stable.The results provided a reference for the screening,culture and further application of Bacillus subtilis var.niger.

关 键 词：枯草芽孢杆菌黑色变种 酪氨酸 选择性培养基 生物指示剂 

分 类 号：Q93-3[生物学—微生物学]