Neural stimulation and modulation with subcellular precision by optomechanical bio-dart  

作　　者：Guoshuai Zhu Jianyun Xiong Xing Li Ziyi He Shuhan Zhong Junlin Chen Yang Shi Ting Pan Li Zhang Baojun Li Hongbao Xin 

机构地区：[1]Guangdong Provincial Key Laboratory of Nanophotonic Manipulation,Institute of Nanophotonics,College of Physics and Optoelectronic Engineering,Jinan University,Guangzhou 511443,China [2]Key Laboratory of CNS Regeneration(Ministry of Education),Guangdong-Hong Kong-Macao Institute of CNS Regeneration,Jinan University,Guangzhou 510632,China

出　　处：《Light(Science & Applications)》2024年第11期2668-2679,共12页光（科学与应用)（英文版）

基　　金：supported by the National Natural Science Foundation of China(12374286,62135005,12204196,and 32271405);the National Key Research and Development Program of China(2023YFF0613700);Guangdong Basic and Applied Basic Research Foundation(2022B1515120012);Science and Technology Program of Guangzhou(202201010370).

摘　　要：Neural stimulation and modulation at high spatial resolution are crucial for mediating neuronal signaling and plasticity,aiding in a better understanding of neuronal dysfunction and neurodegenerative diseases.However,developing a biocompatible and precisely controllable technique for accurate and effective stimulation and modulation of neurons at the subcellular level is highly challenging.Here,we report an optomechanical method for neural stimulation and modulation with subcellular precision using optically controlled bio-darts.The bio-dart is obtained from the tip of sunflower pollen grain and can generate transient pressure on the cell membrane with submicrometer spatial resolution when propelled by optical scattering force controlled with an optical fiber probe,which results in precision neural stimulation via precisely activation of membrane mechanosensitive ion channel.Importantly,controllable modulation of a single neuronal cell,even down to subcellular neuronal structures such as dendrites,axons,and soma,can be achieved.This bio-dart can also serve as a drug delivery tool for multifunctional neural stimulation and modulation.Remarkably,our optomechanical bio-darts can also be used for in vivo neural stimulation in larval zebrafish.This strategy provides a novel approach for neural stimulation and modulation with sub-cellular precision,paving the way for high-precision neuronal plasticity and neuromodulation.

关 键 词：STIMULATION NEURAL precisely 

分 类 号：TH74[机械工程—光学工程]