Development of bi-oligo(ethylene glycol)-functionalized fluorescent probe for two-photon and noninvasive imaging in Arabidopsis thaliana  

作　　者：Jingzhi Du Keming Chen Zhenyang Yu Yuhao Qiao Jiaxin Liu Qianqian Zhai Zhe Hu Sheng-Gang Yang Jun Li Huailong Teng 

机构地区：[1]Department of Chemistry,College of Science,Huazhong Agricultural University,Wuhan,430070,PR China [2]College of Pharmacy,Guizhou Medical University,Guiyang,550025,PR China [3]State Key Laboratory of Agricultural Microbiology,Huazhong Agricultural University,Wuhan,430070,PR China

出　　处：《Advanced Agrochem》2022年第2期162-173,共12页现代农业化学（英文）

基　　金：supported by the National Natural Science Foundation of China(21806048,21801086 and 22071072).

摘　　要：Fluorescent probe is an important tool for investigation of biological events in plant tissues.However,the research on small-molecule based fluorescent probe for plant imaging still stays at the initial stage.In order to noninvasively achieve the subcellular information of Arabidopsis thaliana,a two-photon and fluorene based fluorophore with a bi-oligo(ethylene glycol)functional group was synthesized.Under the laser's irradiation at 750 nm,the subcellular structures of Arabidopsis thaliana including stomata and root tips were clearly observed without slicing,which displayed significant superiorities over traditional single-photon excitation microscopy.Subsequently,the above functionalized fluorophore was further modified with a recognition group(dipicolylamine)to form a smart fluorescent probe(LJTP1).As results,LJTP1 not only can sensitively detect Cu^(2+)/S^(2-)with fluorescence“on-off-on”response in vitro and in vivo,but also can achieve noninvasive imaging in Arabidopsis thaliana tissues under two-photon microscopy.We believe this study will open up a new mind for noninvasive imaging in plant tissues.

关 键 词：Fluorescent probe TWO-PHOTON Plant imaging Dual-functional detection 

分 类 号：Q94[生物学—植物学]