Recent advances in keratinase production via protein engineering,breeding,and fermentation  

作　　者：Ali Raza Ishaq Zheng Zhang Penghui He Min Xiong Shouwen Chen 

机构地区：[1]State Key Laboratory of Biocatalysis and Enzyme Engineering,Environmental Microbial Technology Center of Hubei Province,College of Life Sciences,Hubei University,Wuhan,430062,China

出　　处：《Advanced Agrochem》2024年第3期188-196,共9页现代农业化学（英文）

基　　金：supported by the National Key Research and Development Program of China(2021YFC2100202);Science and Technology Project of Hubei Tobacco Company(027Y2021-023).

摘　　要：The gene editing and synthetic biological tools have led to the implementation of diverse metabolic engineering approaches to enhance the production of specific enzymes.Microbial keratinases can convert keratin wastes into valuable compounds for mankind.Since the market for keratinases cannot be satisfied by production from wild hosts,it is obligatory to develop hosts with high keratinase yields.The intention of this review is to evaluate microbial keratinase advancement through protein engineering,breeding techniques,and fermentation optimization.The main aim of protein engineering is to improve the heat resistance ability and catalytic activity of keratinases by employing mutagenesis methods.Moreover,modifying the expression elements and host engineering are also two unique ways to augment the keratinase yield.Intending to accelerate the production of modified keratinase,this review attempts to highlight the optimization of expression elements,such as promoter engineering,UTR,signal peptide,and codon optimization.Moreover,the approaches of host engineering including strengthening precursor supply,membrane surface engineering,and optimization of secretion pathways were also explained here.Furthermore,it is also essential to optimize the medium composition and fermentation condition for high keratinase yield.This review also addressed the present advancements,difficulties,and tendencies in the field of microbial keratinase production,along with its potential.

关 键 词：KERATINASE Protein engineering Expression elements BREEDING FERMENTATION 

分 类 号：F42[经济管理—产业经济]