高通量测序技术与电镜技术在RNA表观修饰研究中的应用  

Application of high-throughput sequencing technologies and electron microscopy techniques in investigating RNA epigenetics

作　　者：卜芋芬 苏钰莹 荆艳萍[1,2] 林金星[2] BU Yufen;SU Yuying;JING Yanping;LIN Jinxing(State Key Laboratory of Tree Genetics and Breeding,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083;The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学生物科学与技术学院林木遗传育种全国重点实验室,北京100083 [2]北京林业大学生物科学与技术学院树木花卉育种生物工程国家林业和草原局重点实验室,北京100083

出　　处：《电子显微学报》2024年第6期724-732,共9页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.32170689)。

摘　　要：RNA表观遗传学是继DNA表观遗传学和蛋白表观遗传学的发展而兴起的新兴研究领域。已有的研究结果表明,RNA表观遗传修饰对RNA的加工代谢和生物学过程具有调控作用,在生命科学的多个领域已显示出巨大的应用潜力和商业前景。RNA表观遗传修饰的研究有助于进一步阐明生命活动调控的复杂机制。本文将关注于RNA表观遗传修饰研究中各种技术手段的应用,特别聚焦于高通量测序技术和电镜技术在RNA表观修饰研究中的应用情况以及相关局限性,并对这两种技术在未来可能的联合应用提出了展望。RNA epigenetics has emerged as a burgeoning research field following the development of DNA and protein epigenetics.Current investigations have demonstrated that RNA epigenetic modifications play a regulatory role in RNA processing,metabolism,and various biological processes,showcasing significant application and commercial prospects in various fields of life sciences.Studying RNA epigenetic modifications contributes to a deeper understanding of the intricate mechanisms underlying the regulation of biological activities.This article focused on the application of diverse technical methodologies in RNA epigenetic research,with specific emphasis on the utilization of high-throughput sequencing technologies and electron microscopy techniques.Furthermore,this review discussed the limitations associated with these techniques and provided insights into their potential synergistic applications in future research.

关 键 词：RNA表观修饰 高通量测序技术 电镜技术 应用 

分 类 号：Q943[生物学—植物学] Q942Q336