低温长期胁迫下微生物脱氮的内在机制:关注微生物群落  

Internal mechanism of microbial nitrogen removal under prolonged low-temperature stress:A focus on microbial communities

作　　者：赵志勇 唐涛涛 王胤 ZHAO Zhiyong;TANG Taotao;WANG Yin(Southwest Municipal Engineering Design&Research Institute of China,Sichuan Chengdu 610213,China)

机构地区：[1]中国市政工程西南设计研究总院有限公司,四川成都610213

出　　处：《西南给排水》2024年第6期16-23,共8页Southwest Water & Wastewater

摘　　要：低温限制了高寒高海拔地区污水的稳定处理。然而,低温下微生物功能演替的内部机制仍然未知。为了揭示低温影响污水处理的内部机制,采用了16S高通量测序。结果表明,微生物在长时间的低温胁迫下有效去除了污染物(3-8℃)。化学需氧量(COD)、氨氮(NH_(4)^(+)-N)和总氮(TN)的去除效率分别为80.80%-95.18%、87.91%-99.82%和32.67%-69.88%。Nitrospira和Nitrosomonas的相对丰度分别增加了102.50%和276.92%。此外,异养硝基细菌Terrimonas、Rhodobacter和Ferribacterium的相对丰度显着增加,分别增加了34.63%、22.04%和129.64%。这些细菌占优势地位。本研究为长期低温胁迫下微生物脱氮的内部机制提供了新的视角。Low temperature restricts the stable treatment of wastewater in alpine and high-altitude regions.However,the internal mechanism of microbial functional succession at low temperatures remains unknown.To illustrate the internal mechanism by which low temperatures affect wastewater treatment,16S high-throughput were employed.Results revealed that microorganisms effectively removed pollu-tants under prolonged low-temperature stress(3-8℃).The removal efficiencies for?chemical oxygen demand,NH_(4)^(+)-N,and total nitrogen were 80.80%-95.18%,87.91%-99.82%,and 32.67%-69.88%,respectively.The relative abundance of Nitrospira and Nitrosomonas increased by 102.50% and 276.92%,respectively.Additionally,the relative abundance of heterotrophic nitrobacteria,including Terrimonas,Rhodobacter,and Ferribacterium,increased significantly,with values increasing by 34.63%,22.04%,and 129.64%,respectively.These bacteria became dominant.This study provides novel perspectives on the internal mechanism of microbial nitrogen removal under prolonged low-temperature stress.

关 键 词：低温 高寒高海拔 异养反硝化菌 高通量测序 

分 类 号：X70[环境科学与工程—环境工程]