儿童睡眠呼吸障碍相关的肠道菌群改变与儿童非酒精性脂肪肝病的发病机制探索  

Changes in intestinal flora associated with childhood sleep-disordered breathing and the pathogenesis of non-alcoholic fatty liver disease in children

作　　者：杨小楠 华红婷 汪东[1] 司冬雨 甘芮嘉 孟冬冬 高潮兵[1] YANG Xiaonan;HUA Hongting;WANG Dong;SI Dongyu;GAN Ruijia;MENG Dongdong;GAO Chaobing(Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Anhui Medical University,Hefei,230000,China;Department of Otorhinolaryngology Head and Neck Surgery, Fuyang Women and Children's Hospital)

机构地区：[1]安徽医科大学第一附属医院耳鼻咽喉头颈外科,合肥230000 [2]阜阳市妇女儿童医院耳鼻咽喉头颈外科

出　　处：《临床耳鼻咽喉头颈外科杂志》2024年第11期1038-1044,共7页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:探讨儿童睡眠呼吸障碍(sleep-disordered breathing,SDB)、肠道菌群与儿童非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)之间的相互作用。方法:共纳入63例非肥胖儿童(试验组47例合并SDB,对照组16例未合并SDB的)参与本研究。分别评估2组儿童的肝功能状态和SDB程度。采用高通量16S rRNA测序来检测2组儿童的肠道菌群组成和功能变化。结果:与对照组比较,试验组儿童的血清谷草转氨酶、谷丙转氨酶增高;肠道菌群的变形菌门相对丰度增加,拟杆菌门相对丰度显著减少;肠道菌群的膜运输、碳水化合物代谢、脂质代谢等功能富集。结论:2组儿童肝功能状态和肠道菌群的组成、功能注释存在显著差异。儿童SDB相关肠道菌群的变化可能与儿童NAFLD的发病机制相关。Objective:To explore the interaction between pediatric sleep-disordered breathing(SDB),the intestinal microbiota,and pediatric non-alcoholic fatty liver disease(NAFLD).Methods:A total of 63 non-obese children(47 children with SDB in the experimental group and 16 without SDB in the control group) were enrolled in this study.The liver function and degree of SDB were assessed in both groups.High-throughput 16S rRNA sequencing was conducted to detect the composition and functional variations of the intestinal microbiota in the two groups of children.Results:Compared with children in the experimental group,serum ALT and AST levels were higher in the control group.and the relative proteobacteria abundance of intestinal flora increased,and the relative abundance of Bacteroidetes decreased significantly.Function including membrane transport,carbohydrate metabolism and lipid metabolism,were enriched in the intestinal microbiota of children with SDB.Conclusion:The composition and functional annotation of the pediatric liver functional status and gut microbiota were significantly different between the two groups of children with and without SDB.Changes in SDB-associated intestinal bacterial abundance may be related to the pathogenesis of pediatric NAFLD.

关 键 词：儿童睡眠呼吸障碍 儿童非酒精性脂肪肝病 肠道菌群 

分 类 号：R766[医药卫生—耳鼻咽喉科]