半夏泻心汤对脓毒症大鼠回肠黏膜通透性的影响  

Effect of Banxia Xiexin Decoction on Permeability of Ileum Mucosa in Sepsis Rats

作　　者：牟海波 梁浩 巴特金 张双林 孔立[3] MOU Haibo;LIANG Hao;Batejin;ZHANG Shuanglin;KONG Li(College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Shandong Province,Jinan 250355,China;Emergency and Critical Care Center,Inner Mongolia Autonomous Region International Mongolian Medical Hospital,Inner Mongolia,Hohhot 010010,China;Emergency and Critical Care Center,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Shandong Province,Jinan 250011,China)

机构地区：[1]山东中医药大学中医学院,山东济南250355 [2]内蒙古自治区国际蒙医医院急诊与重症医学中心,内蒙古呼和浩特010010 [3]山东中医药大学附属医院急诊与重症医学中心,山东济南250011

出　　处：《中国中医药现代远程教育》2024年第23期162-165,共4页Chinese Medicine Modern Distance Education of China

基　　金：国家自然科学基金【No.82204876】;山东省自然科学基金【No.ZR2020MH347,ZR2020QH308】。

摘　　要：目的研究半夏泻心汤对脓毒症大鼠回肠黏膜通透性的影响。方法将45只雄性SD大鼠随机分为假手术组、脓毒症组和半夏泻心汤组,假手术组开腹后关腹,脓毒症组和半夏泻心汤组采用盲肠结扎穿孔法制备脓毒症大鼠模型。半夏泻心汤组术后予10 mL/kg半夏泻心汤灌胃,假手术组、脓毒症组予10 mL/kg的0.9%氯化钠注射液灌胃。成模24 h后,留取血清检测促炎因子[肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-17、D-乳酸、二胺氧化酶(DAO)、内毒素]水平变化,留取回肠组织标本观察光镜下的病理变化。结果半夏泻心汤组大鼠TNF-α、IL-1β、IL-17、D-乳酸、DAO、内毒素水平明显低于脓毒症组(P<0.05),回肠组织病理学评分亦低于脓毒症组(P<0.05)。结论半夏泻心汤通过调控炎症反应,有效降低了脓毒症大鼠的回肠通透性,减轻了回肠的病理改变。Objective To study on the effect of Banxia Xiexin decoction on permeability of ileum mucosa in sepsis rats.Methods Forty-five male SD rats were randomly divided into the sham-operated group,the sepsis group and Banxia Xiexin decoction group.In the sham-operated group,the abdomen was opened and then closed,and the sepsis model was prepared by cecum ligation and perforation.In Banxia Xiexin decoction group,10 ml/kg of Banxia Xiexin decoction was given by gastric gavage after the operation,in pseudo-operation and sepsis group,10 ml/kg of saline was given by gastric gavage.After 24h of modeling,serum was retained to detect the changes of pro-inflammatory factors tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-17(IL-17),Dlactic acid,diamine oxidase(DAO),and endotoxin,and ileal tissue specimens were retained for the observation of pathological changes of ileal tissues under light microscopy.Results TNF-α,IL-1β,and IL-17 in Banxia Xiexin decoction group were significantly lower than that in the sepsis group(P<0.01),and D-lactic acid,DAO,and endotoxin were lower than in the sepsis group(P<0.05),histopathological scores of the ileum in the Banxia Xiexin decoction group were lower than those in the sepsis group(P<0.05).Conclusion Banxia Xiexin decoction effectively reduces ileal permeability and attenuates pathological changes in the ileum of septic rats by modulating the inflammatory response.

关 键 词：脓毒症 半夏泻心汤 炎症反应 肠黏膜通透性 实验研究 

分 类 号：R285.5[医药卫生—中药学]