DIECA对小麦雄性育性的影响  

Effects of DIECA on wheat male fertility

作　　者：刘海英[1] 孔陈源 茹振钢[2] 董娜[2] 陈向东[2] 陈芸 黄康兴 黄紫薇 聂莹莹 Liu Haiying;Kong Chenyuan;Ru Zhengang;Dong Na;Chen Xiangdong;Chen Yun;Huang Kangxing;Huang Ziwei;Nie Yingying(College of Life Sciences,Henan Normal University,Xinxiang 453007,China;Center of Wheat Research,Henan Institute of Science and Technology,Xinxiang 453003,China)

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]河南科技学院小麦中心,河南新乡453003

出　　处：《河南师范大学学报（自然科学版）》2025年第1期32-42,I0002-I0004,共14页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31701502);河南省科技厅科技攻关项目(222102110216);河南省重大科技专项(221100110300).

摘　　要：为探究DIECA(sodiumdiethyldithiocarbamate,二乙基二硫代氨基甲酸钠)对小麦雄性育性的影响,以小麦温敏雄性不育系BNS366及其近等基因系郑麦366为试验材料,在正常秋季播种(2021-10-13)和晚播(2021-12-01)条件下,于2022年春季小麦雌雄蕊原基分化期至开花期采用酶联免疫法测定幼穗和花药中内源MeJA含量,采用0(清水,CK)、0.1、1、5、10、20和30 mmol·L^(-1)DIECA水溶液对小麦进行全株喷施处理,用I 2-KI法测定花粉可育率;用国内法和国际法测定自交结实率.结果表明:在正常秋季播种条件下,在花粉母细胞期后,郑麦366内源MeJA含量出现了刺激性增长,BNS366则出现不足现象;1 mmol·L^(-1)和5 mmol·L^(-1)DIECA处理对正常秋季播种郑麦366的自交结实率具有显著降低效应,5 mmol·L^(-1)DIECA处理效应较强,其国内法自交结实率和国际自交结实率分别比对照降低了39.82%和44.68%,在不同播期下,郑麦366和BNS366其他DIECA处理的花粉可育率、国内法自交结实率和国际法自交结实率与对照相比差异均不显著.DIECA可能通过抑制郑麦366内源MeJA含量的刺激性增长,影响花药发育和正常开裂,降低了郑麦366的雄性育性,内源MeJA可能参与了BNS366雄性不育的发生.In order to investigate the effects of sodium diethyldithiocarbamate(DIECA)on wheat male fertility,the thermo-sensitive genic male sterile wheat BNS366 and its near-isogenic line Zhengmai366 were used as materials.The two materials were sown under conditons of normal sowing in autumn(2021-10-13)and late sowing(2021-12-01),respectively.In the spring of 2022,indirect enzyme-linked immunosorbent assays method was used to endogenous methyl jasmonate(MeJA)content in young panicles and anthers from the differentiation stage of pistil and stamen primordium to anthesis,and the wheat was sprayed with 7 different concentrations(0(water/CK)、0.1、1、5、10、20 and 30 mmol·L^(-1))of DIECA solution.I 2-KI method was used to detect the frequency of fertile pollen grains at anthesis stage.National and international methods were used to determine the self-seeds rate.The results showed that under the condition of normal sowing in autumn,after the pollen mother cell stage,there was stimulating growth in the content of endogenous MeJA in Zhengmai366,however,there was shortage in BNS366.Under the condition of normal sowing in autumn,sprayed with 1 mmol·L^(-1)DIECA and 5 mmol·L^(-1)DIECA the self-seeds rate of Zhengmai366 were decreased which meant the male fertility of Zhengmai366 was decreased,and the treatment of 5 mmol·L^(-1)DIECA had stronger effect on it,i.e.,the self-seeds rates by national and international methods were reduced by 39.82%and 44.68%respectively compared with the control group.the frequency of fertile pollen grains and the self-seeds rates by national and international methods of Zhengmai366 and BNS366 under other DIECA treatments were not significantly different from the control.It was probable that DIECA inhibited the stimulating growth above mentioned,that affected the anther development and dehiscence regularly that decreased the male fertility of Zhengmai366,and the endogenous MeJA might involved the occurrence of male sterility in BNS366.

关 键 词：小麦 DIECA 雄性育性 

分 类 号：S512[农业科学—作物学] S330