娑罗子中七叶皂苷A的超声-微波协同提取工艺优化  

Optimization of Ultrasound-Microwave Synergistic Extraction of Aescin A from Aesculi Semen

作　　者：王佳颖 王佰灵 罗婷 刘雨蒙 戈振凯 WANG Jiaying;WANG Bai-ling;LUO Ting;LIU Yu-meng;GE Zhen-kai(School of Pharmaceutical Sciences,Hubei Key Laboratory of Wudang Local Chinese Medicine Research;Department of Clinical Laboratory,Taihe Hospital,Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]湖北医药学院药学院武当特色中药研究湖北省重点实验室 [2]十堰市太和医院·湖北医药学院附属医院检验科,湖北十堰442000

出　　处：《湖北医药学院学报》2024年第6期596-601,606,共7页Journal of Hubei University of Medicine

基　　金：湖北省大学生创新创业项目(S202310929019X);湖北医药学院研究生启动金(2013QDJZR13)。

摘　　要：目的:采用Box-Behnken响应面法(RSM)优化娑罗子中七叶皂苷A的超声-微波协同提取工艺。方法:采用HPLC法测定七叶皂苷A的含量,单因素试验考察提取溶剂、料液比、超声功率、微波功率、提取温度、提取时间对七叶皂苷A提取率的影响,Box-Behnken响应面法优化超声-微波协同提取工艺。结果:优化所得最佳提取工艺为:提取溶剂40%乙醇(V/V),料液比1∶34(g/mL),超声功率230 W,微波功率200 W,提取温度40℃,提取时间10 min。在此工艺条件下对三批次药材进行验证,提取率为0.7625%,与模型预测值0.7587%接近,表明所建模型可靠有效。结论:超声-微波协同提取工艺提取时间短、提取率高、稳定可靠且绿色环保,可为工业上娑罗子中七叶皂苷A的提取提供参考。Objective To optimize the ultrasound-microwave synergitic extraction of Aescin A from Aesculi Semen using the Box-Behnken response surface methodology(RSM).Methods The content of Aescin A was determined by HPLC.Single factor tests were conducted to investigate the effects of extraction solvent,solid-liquid ratio,ultrasonic power,microwave power,extraction temperature,and extraction time on the extraction of Aescin A.RSM was used to optimize the ultrasound-microwave synergistic extraction process.Results The optimal extraction process was as follows:extraction solvent of 40%ethanol(V/V),solid-liquid ratio at 1∶34(g/mL),ultrasonic power of 230 W,microwave power of 200 W,extraction temperature of 40℃,and extraction time of 10 min.Under this condition,the extraction rate of Aescin A from Semen Aesculi of three batches was 0.7625%,which was close to the predicted 0.7587%,indicating the reliability and effectiveness of the model.Conclusion Ultrasound-microwave synergistic extraction has the advantages of short extraction time,high extraction rate,stability,reliability,and environmental protection,which provides a certain reference for the extraction of Aescin A from Aesculi Semen in industrial applications.

关 键 词：娑罗子 七叶皂苷A 超声-微波协同提取 

分 类 号：R284.2[医药卫生—中药学]